WST-8
Based on 17 publication(s) in Google Scholar
WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.
For research use only. We do not sell to patients.
- Purity: 99.69%
- CAS No.: 193149-74-5
- Formula: C20H13N6NaO11S2
- Molecular Weight:600.47
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) WST-8
More- Nat Commun. 2022 Oct 12;13(1):6016. [Abstract]
- Biomaterials. 2026 Feb:325:123623. [Abstract]
- Sci China Life Sci. 2022 Mar;65(3):618-630. [Abstract]
- J Med Chem. 2025 May 22;68(10):10410-10424. [Abstract]
- J Med Chem. 2024 Sep 26;67(18):16598-16611. [Abstract]
- Talanta. 2025 Nov 25:300:129159. [Abstract]
- RSC Adv. 2020 Dec 9;10(71):43480-43488. [Abstract]
- Cells. 2026 May 14;15(10):898. [Abstract]
- Microorganisms. 2021 Mar 31;9(4):726. [Abstract]
- Res Microbiol. 2023 Sep-Oct;174(7):104090. [Abstract]
- J Nat Prod. 2023 Jan 27;86(1):8-17. [Abstract]
- J Phys Chem B. 2023 May 18;127(19):4277-4285. [Abstract]
- Int J Clin Exp Pathol. 2020 Oct 1;13(10):2474-2486. [Abstract]
- bioRxiv. 2025 Sep 30.
- Preprints. 2025 May 07.
- University of California, Berkeley. 2024.
- Res Sq. 2024 Jul 11.
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation
1.1 Preparation of stock solution
Solvent: H2O
Concentration: 50 mM is recommended.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 5 mM with cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining
2.1 Cells are seeded in a 96-well plate at a concentration of 1.0-2.0?×?103 cells/well/100μL
2.2 Add 10 μL WST-8 at a final concentration of 5 mM.
2.3 Incubate cells at room temperature or 37°C for 1-4?h.
2.4 The absorbance at 450?nm was determined with a microplate reader.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 193149-74-5
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Appearance Solid
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Molecular Weight 600.47
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Formula C20H13N6NaO11S2
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Color White to yellow
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SMILES
COC1=CC([N+]([O-])=O)=CC=C1[N+]2=NC(C3=CC=C(C=C3S(=O)([O-])=O)S(=O)(O[Na])=O)=NN2C4=CC=C(C=C4)[N+]([O-])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)
Publications (17)
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Journal Impact Factor
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Most Recent
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Nat Commun
Structural insight into the bulge-containing KRAS oncogene promoter G-quadruplex bound to berberine and coptisine. [Abstract]2022 Oct 12;13(1):6016. PMID: 36224201 -
Biomaterials
A novel Kupffer cell-targeting nanoparticle system to Mitigate alcohol-associated liver disease. [Abstract]2026 Feb:325:123623. PMID: 40795708 -
Sci China Life Sci
Characterization of Photorhabdus Virulence Cassette as a causative agent in the emerging pathogen Photorhabdus asymbiotica. [Abstract]2022 Mar;65(3):618-630. PMID: 34185241 -
J Med Chem
2025 May 22;68(10):10410-10424. PMID: 40336482 -
J Med Chem
2024 Sep 26;67(18):16598-16611. PMID: 39236094 -
Talanta
Colorimetric determination of the plant toxin β-cyano-l-alanine using a multistep enzyme reaction. [Abstract]2025 Nov 25:300:129159. PMID: 41338014 -
RSC Adv
Phase controlled one-pot synthesis of heterostructured FePt-Fe3O4 nanocubes with excellent biocompatibility. [Abstract]2020 Dec 9;10(71):43480-43488. PMID: 35519686 -
Cells
2026 May 14;15(10):898. PMID: 42193905 -
Microorganisms
New Insights into the Virulence Traits and Antibiotic Resistance of Enterococci Isolated from Diverse Probiotic Products. [Abstract]2021 Mar 31;9(4):726. PMID: 33807433 -
Res Microbiol
Bacillus pfraonensis sp. nov., a new strain isolated from a probiotic feed additive with low cytotoxicity and antimicrobial activity. [Abstract]2023 Sep-Oct;174(7):104090. PMID: 37356781 -
J Nat Prod
1H NMR-Based Biochemometric Analysis of Morus alba Extracts toward a Multipotent Herbal Anti-Infective. [Abstract]2023 Jan 27;86(1):8-17. PMID: 36543521 -
J Phys Chem B
2023 May 18;127(19):4277-4285. PMID: 37140453 -
Int J Clin Exp Pathol
Circular RNA MGAT1 regulates cell proliferation and apoptosis in hypoxia-induced cardiomyocytes through miR-34a/YAP1 axis. [Abstract]2020 Oct 1;13(10):2474-2486. PMID: 33165436 -
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Solvent & Solubility
H2O : 80 mg/mL (133.23 mM; ultrasonic and warming and heat to 60°C)
DMSO : 10 mg/mL (16.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.6654 mL | 8.3268 mL | 16.6536 mL | 41.6341 mL |
| 5 mM | 0.3331 mL | 1.6654 mL | 3.3307 mL | 8.3268 mL | |
| 10 mM | 0.1665 mL | 0.8327 mL | 1.6654 mL | 4.1634 mL | |
| 15 mM | 0.1110 mL | 0.5551 mL | 1.1102 mL | 2.7756 mL | |
| H2O | 20 mM | 0.0833 mL | 0.4163 mL | 0.8327 mL | 2.0817 mL |
| 25 mM | 0.0666 mL | 0.3331 mL | 0.6661 mL | 1.6654 mL | |
| 30 mM | 0.0555 mL | 0.2776 mL | 0.5551 mL | 1.3878 mL | |
| 40 mM | 0.0416 mL | 0.2082 mL | 0.4163 mL | 1.0409 mL | |
| 50 mM | 0.0333 mL | 0.1665 mL | 0.3331 mL | 0.8327 mL | |
| 60 mM | 0.0278 mL | 0.1388 mL | 0.2776 mL | 0.6939 mL | |
| 80 mM | 0.0208 mL | 0.1041 mL | 0.2082 mL | 0.5204 mL | |
| 100 mM | 0.0167 mL | 0.0833 mL | 0.1665 mL | 0.4163 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
WST-8 Related Classifications
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