Stauntoside Ⅱ
Stauntoside Ⅱ is a 14,15-secopregnane-type C21 steroidal glycoside found in the roots of Cynanchum stauntonii. Stauntoside Ⅱ does not exhibit cytotoxic activity against human colon, hepatoma, gastric, lung, and ovarian cancer cells.
For research use only. We do not sell to patients.
- CAS No.: 2183489-10-1
- Formula: C27H38O7
- Molecular Weight:474.59
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Stauntoside Ⅱ (compound 4) (24 h) shows no cytotoxic activity against HCT-8, Bel-7402, BGC-823, A549 and A2780 human tumor cell lines[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:human colon cancer cell line HCT-8, human hepatoma cancer cell line Bel-7402, human gastric cancer cell line BGC-823, human lung cancer cell line A549, human ovarian cancer cell line A2780
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Concentration:/
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Incubation Time:24 h
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Result:Exhibited no cytotoxic activity against the five tested human tumor cell lines, with an IC50 value greater than 10 μM.
Chemical Information
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CAS No. 2183489-10-1
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Molecular Weight 474.59
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Formula C27H38O7
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SMILES
C[C@@]12[C@]3([H])[C@]4(CO2)C(O[C@]3([H])CO1)=C5[C@]([C@@]6(C(C[C@H](CC6)O[C@H]7C[C@H]([C@@H]([C@H](O7)C)O)O)=CC5)C)([H])CC4
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Liver Cancer Modeling
Liver cancer can be classified into primary liver cancer and secondary liver cancer. Secondary liver cancer is the metastatic liver cancer. Primary liver cancer includes hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and fibrolamellar HCC, of which HCC is the most common form, accounting for approximately 90% of primary liver cancers[1]. HCC mouse models include chemical agent-induced models, transplanted tumor models, and genetic engineered models.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Stauntoside Ⅱ Related Classifications
HY-N18228 Related Classifications
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