Tositumomab
Based on 1 Customer Validation
Tositumomab (Anti-Human CD20 Recombinant Antibody) is a mouse-derived IgG2a monoclonal antibody, targeting to human CD20. Tositumomab can deplete B cells. Tositumomab can be used for the research of cancer, such as lymphoma.
For research use only. We do not sell to patients.
- Purity: 96.00%
- CAS No.: 192391-48-3
- Molecular Weight:147.298 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Description
Isotype
Mouse IgG2a
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
MS4A1
In Vitro
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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IgG2a-lambda
Application
ELISA, FACS, Functional assay
Verified Bioactivity
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Flow Cytometry analysis of Raji cells labelling CD20/MS4A1 (red) with Tositumomab (anti-CD20/MS4A1) (HY-P99326). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG2a (HY-P99978, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black). -
Loaded Tositumomab on AHC2 biosensor, can bind CD20/MS4A1 Protein, Human (Trx-His, HY-P7896A) with an affinity constant of <1.000E-11 M as determined in BLI assay.
Chemical Information
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CAS No. 192391-48-3
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Appearance Liquid
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Molecular Weight 147.298 kDa
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Color Colorless to light yellow
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SMILES
[Tositumomab]
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Synonyms
Anti-Human CD20 Recombinant Antibody; V10XA53
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (257 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Havlena GT, et al. Cure of Micrometastatic B-Cell Lymphoma in a SCID Mouse Model Using 213Bi-Anti-CD20 Monoclonal Antibody. J Nucl Med. 2023 Jan;64(1):109-116. [Content Brief]
[2]. Beers SA, et al. Type II (tositumomab) anti-CD20 monoclonal antibody out performs type I (rituximab-like) reagents in B-cell depletion regardless of complement activation. Blood. 2008 Nov 15;112(10):4170-7. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)