Janus green B
Based on 1 Customer Validation
Janus green B is a supravital stain. Janus green B staining reaction is oxygen dependent, and is reversibly inhibited by cyanide. Janus green B has been used for staining peripheral nerves in live insects, lymphatic vessels of rabbits and mitochondria.
For research use only. We do not sell to patients.
- Purity: 98.75%
- CAS No.: 2869-83-2
- Formula: C30H31ClN6
- Molecular Weight:511.06
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Observing New Linear Structures inside Rabbit Lymphatic Vessels using Janus Green B Staining Method[1]
(1) Preparation of experimental animals: Anesthesia is administered to 12-week-old New Zealand rabbits, and an incision is made on the left side or in the middle of the rabbit's abdomen to expose the large lymphatic vessel located next to the inferior vena cava.
(2) Dissolve Janus Green B in DMSO with a dye content of 65%.
(3) Staining: Using a 30.5-gauge needle, approximately 0.3 milliliters of the preheated Janus Green B staining solution is injected as slowly as possible into a lymph node or lymphatic vessel close to the inferior vena cava. The injection needle is gently withdrawn, and the flow of the Janus Green B staining solution in the lymphatic vessel is observed presenting a blue color.
(4) Washing and maintaining temperature: A preheated phosphate-buffered saline (pH 7.4) at 40°C is injected to remove any backflow of the Janus Green B staining solution and to maintain the tissue's temperature and active state.
(5) Observation and localization: The internal condition of the lymphatic vessels is carefully observed using a stereomicroscope to determine the location of well-stained areas. The focus of observation is adjusted according to the staining effect and individual differences.
(6) Fixing samples: The collected samples are immersed in a 10% neutral formalin solution for fixation as soon as possible.
(7) Additional staining (optional): A portion of the fixed samples is selected for secondary staining using a 0.1% (w/v) acridine orange PBS solution.
(8) Imaging: Confocal laser scanning microscopy is used for imaging analysis of the stained samples.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2869-83-2
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Appearance Solid
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Molecular Weight 511.06
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Formula C30H31ClN6
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Color Brown to black
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SMILES
CN(C1=CC=C(/N=N/C2=CC3=[N+](C4=CC=CC=C4)C5=C(C=CC(N(CC)CC)=C5)N=C3C=C2)C=C1)C.[Cl-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 116.67 mg/mL (228.29 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Lee BC, et al. Novel threadlike structures (Bonghan ducts) inside lymphatic vessels of rabbits visualized with a Janus Green B staining method. Anat Rec B New Anat. 2005 Sep;286(1):1-7. [Content Brief]
[2]. LAZAROW A, et al. Studies on the enzymatic basis for the Janus green B staining reaction. J Histochem Cytochem. 1953 Jul;1(4):234-41. [Content Brief]
[3]. Yack JE. Janus Green B as a rapid, vital stain for peripheral nerves and chordotonal organs in insects. J Neurosci Methods. 1993 Aug;49(1-2):17-22. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9567 mL | 9.7836 mL | 19.5672 mL | 48.9179 mL |
| 5 mM | 0.3913 mL | 1.9567 mL | 3.9134 mL | 9.7836 mL | |
| 10 mM | 0.1957 mL | 0.9784 mL | 1.9567 mL | 4.8918 mL | |
| 15 mM | 0.1304 mL | 0.6522 mL | 1.3045 mL | 3.2612 mL | |
| 20 mM | 0.0978 mL | 0.4892 mL | 0.9784 mL | 2.4459 mL | |
| 25 mM | 0.0783 mL | 0.3913 mL | 0.7827 mL | 1.9567 mL | |
| 30 mM | 0.0652 mL | 0.3261 mL | 0.6522 mL | 1.6306 mL | |
| 40 mM | 0.0489 mL | 0.2446 mL | 0.4892 mL | 1.2229 mL | |
| 50 mM | 0.0391 mL | 0.1957 mL | 0.3913 mL | 0.9784 mL | |
| 60 mM | 0.0326 mL | 0.1631 mL | 0.3261 mL | 0.8153 mL | |
| 80 mM | 0.0245 mL | 0.1223 mL | 0.2446 mL | 0.6115 mL | |
| 100 mM | 0.0196 mL | 0.0978 mL | 0.1957 mL | 0.4892 mL |
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