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  4. Janus green B

Janus green B is a supravital stain. Janus green B staining reaction is oxygen dependent, and is reversibly inhibited by cyanide. Janus green B has been used for staining peripheral nerves in live insects, lymphatic vessels of rabbits and mitochondria.

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Janus green B Chemical Structure

Janus green B Chemical Structure

CAS No. : 2869-83-2

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Janus green B Related Antibodies

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Description

Janus green B is a supravital stain. Janus green B staining reaction is oxygen dependent, and is reversibly inhibited by cyanide. Janus green B has been used for staining peripheral nerves in live insects, lymphatic vessels of rabbits and mitochondria[1][2][3].

Molecular Weight

511.06

Formula

C30H31ClN6
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

CN(C1=CC=C(/N=N/C2=CC3=[N+](C4=CC=CC=C4)C5=C(C=CC(N(CC)CC)=C5)N=C3C=C2)C=C1)C.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (244.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9567 mL 9.7836 mL 19.5672 mL
5 mM 0.3913 mL 1.9567 mL 3.9134 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9567 mL 9.7836 mL 19.5672 mL 48.9179 mL
5 mM 0.3913 mL 1.9567 mL 3.9134 mL 9.7836 mL
10 mM 0.1957 mL 0.9784 mL 1.9567 mL 4.8918 mL
15 mM 0.1304 mL 0.6522 mL 1.3045 mL 3.2612 mL
20 mM 0.0978 mL 0.4892 mL 0.9784 mL 2.4459 mL
25 mM 0.0783 mL 0.3913 mL 0.7827 mL 1.9567 mL
30 mM 0.0652 mL 0.3261 mL 0.6522 mL 1.6306 mL
40 mM 0.0489 mL 0.2446 mL 0.4892 mL 1.2229 mL
50 mM 0.0391 mL 0.1957 mL 0.3913 mL 0.9784 mL
60 mM 0.0326 mL 0.1631 mL 0.3261 mL 0.8153 mL
80 mM 0.0245 mL 0.1223 mL 0.2446 mL 0.6115 mL
100 mM 0.0196 mL 0.0978 mL 0.1957 mL 0.4892 mL
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Janus green B Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Janus green B2869-83-2Fluorescent Dyesupravitalstainoxygen dependentInhibitorinhibitorinhibit

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