Cat. No.: HY-118704

P-2281

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Based on 1 Customer Validation

P-2281 is a mTOR inhibitor with anticancer and anti-inflammatory efficacies. P2281 inhibits mTOR activity in colon cancer cells. P-2281 suppresses Dextran sulfate sodium salt (HY-116282C) (DSS)-induced colitis by inhibiting T cell function and is efficacious in a murine model of human colitis.

For research use only. We do not sell to patients.

Purity: 99.81%
CAS No.: 1112994-35-0
Formula: C₉H₈ClN₃O
Molecular Weight:209.63

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

P-2281 Chemical Structure

CAS No.: 1112994-35-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All mTOR Isoforms :

In Vitro

P2281 (30 μM) diminishes induced IFN-γ production but not TNF-αproduction in human peripheral blood mononuclear cells (hPBMCs)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

P2281 (15 mg/kg; ip; daily; for 10 days) suppresses DSS-induced colitis by inhibiting T cell function^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6J mice (6 wk of age, weighing 18-22 g) by giving 3% (wt/vol) DSS in drinking water^[1].
Dosage:

15 mg/kg
Administration:

ip; daily; for 10 days
Result:

Significantly inhibited DSS-induced weight loss, improved rectal bleeding index, decreased disease activity index, and reversed DSS-induced shortening of the colon.
Distinctly attenuated DSS-induced edema, prominently diminished the leukocyte infiltration in the colonic mucosa.
Blocked DSS-induced activation of mTOR.

Chemical Information

CAS No. 1112994-35-0
Appearance Solid
Molecular Weight 209.63
Formula C₉H₈ClN₃O
Color White to off-white
SMILES

CC(C(NC1=CC=C(N=C1)C#N)=O)Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (596.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.7703 mL

23.8515 mL

47.7031 mL

5 mM

0.9541 mL

4.7703 mL

9.5406 mL

10 mM

0.4770 mL

2.3852 mL

4.7703 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Mandar R Bhonde, et al. A novel mTOR inhibitor is efficacious in a murine model of colitis. Am J Physiol Gastrointest Liver Physiol. 2008 Dec;295(6):G1237-45. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.7703 mL	23.8515 mL	47.7031 mL	119.2577 mL
	5 mM	0.9541 mL	4.7703 mL	9.5406 mL	23.8515 mL
	10 mM	0.4770 mL	2.3852 mL	4.7703 mL	11.9258 mL
	15 mM	0.3180 mL	1.5901 mL	3.1802 mL	7.9505 mL
	20 mM	0.2385 mL	1.1926 mL	2.3852 mL	5.9629 mL
	25 mM	0.1908 mL	0.9541 mL	1.9081 mL	4.7703 mL
	30 mM	0.1590 mL	0.7951 mL	1.5901 mL	3.9753 mL
	40 mM	0.1193 mL	0.5963 mL	1.1926 mL	2.9814 mL
	50 mM	0.0954 mL	0.4770 mL	0.9541 mL	2.3852 mL
	60 mM	0.0795 mL	0.3975 mL	0.7951 mL	1.9876 mL
	80 mM	0.0596 mL	0.2981 mL	0.5963 mL	1.4907 mL
	100 mM	0.0477 mL	0.2385 mL	0.4770 mL	1.1926 mL

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P-2281

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

PI3K/Akt/mTOR Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Reprogramming Compound Library

Oxygen Sensing Compound Library

Glycolysis Compound Library

Cytoskeleton Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Glucose Metabolism Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Mitochondrial Protection Compound Library

Cysteine Targeted Covalent Library

Cysteine Targeted Covalent Fragment Library

Serine/Threonine Kinase Inhibitor Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

Anti-Brain Cancer Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Lactylation Compound Library

All mTOR Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

P-2281 Related Classifications

HY-118704 Related Classifications