QTZ
Based on 1 Customer Validation
QTZ is a bioluminescence agent for in vivo imaging. QTZ has red-shifted emission and yields very little background. QTZ is a coelenterazine analog with the 4-quinolinyl substitution at the C8 position of the imidazopyrazinone core.
For research use only. We do not sell to patients.
- Purity: 96.0%
- CAS No.: 2883232-39-9
- Formula: C28H20N4O
- Molecular Weight:428.48
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be adjusted according to your specific needs).
1. Solution preparation[1]
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 25 mM (optimized according to the experiment).
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 25-50 μM with DPBS or other buffers.
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining
HeLa and MDA-MB-453 cells, and dilute to 1 × 105 cells/mL with DPBS.
10 μg of purified scFV-QLuc fusion protein was added to 1 mL of cell suspension and incubated on ice for 30 min.
Cells were pelleted by centrifugation and resuspended in 1 mL of DPBS.
100 μL of cell suspension was added to a 96-well plate.
100 μL of 50 μM QTZ in DPBS was added to the cell suspension to a final concentration of 25 μM.
Bioluminescence intensity was recorded using a microplate reader equipped with an extended range red-sensitive photomultiplier tube.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Anesthetize the Her2+ tumor.
The scFv-QLuc fusion protein (1 mg) diluted with normal saline to a volume of 100 μL was administered through the tail vein.
6 hours later, intravenously inject 100 μL QTZ (1 mM) (25% (w/v) HP-β-CD and 20% PEG-400 dissolved in normal saline).
Bioluminescence imaging is performed immediately after substrate administration in the aforementioned Bioluminescence imaging dark box.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2883232-39-9
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Appearance Solid
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Molecular Weight 428.48
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Formula C28H20N4O
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Color Yellow to orange
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SMILES
O=C1C(CC2=CC=CC=C2)=NC3=C(NC(C4=CC=CC=C4)=CN13)C5=C6C=CC=CC6=NC=C5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : ≥ 25 mg/mL (58.35 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3338 mL | 11.6692 mL | 23.3383 mL | 58.3458 mL |
| 5 mM | 0.4668 mL | 2.3338 mL | 4.6677 mL | 11.6692 mL | |
| 10 mM | 0.2334 mL | 1.1669 mL | 2.3338 mL | 5.8346 mL | |
| 15 mM | 0.1556 mL | 0.7779 mL | 1.5559 mL | 3.8897 mL | |
| 20 mM | 0.1167 mL | 0.5835 mL | 1.1669 mL | 2.9173 mL | |
| 25 mM | 0.0934 mL | 0.4668 mL | 0.9335 mL | 2.3338 mL | |
| 30 mM | 0.0778 mL | 0.3890 mL | 0.7779 mL | 1.9449 mL | |
| 40 mM | 0.0583 mL | 0.2917 mL | 0.5835 mL | 1.4586 mL | |
| 50 mM | 0.0467 mL | 0.2334 mL | 0.4668 mL | 1.1669 mL |
QTZ Related Classifications
HY-47176 Related Classifications
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