AMPPD
Based on 1 publication(s) in Google Scholar
AMPPD (Lumi-Phos Plus; Lumigen PPD) is a chemiluminescent substrate for alkaline phosphatase (APase). AMPPD is hydrolyzed by APase to generate an unstable dioxetane intermediate, and the intermediate releases a chemiluminescent signal when it decomposes. The luminescent signal of AMPPD can be detected by highly sensitive equipment, thereby achieving quantitative analysis of the target molecule. AMPPD can be used in ultrasensitive enzyme-linked immunosorbent assays (such as quantitative detection of human tissue kininogen), chemiluminescent detection of proteins and nucleic acids, and other fields.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.29%
- CAS 番号: 122341-56-4
- 分子式: C18H23O7P
- 分子量:382.34
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 AMPPD
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生物活性
AMPPD (0.4 mM; 20 min) can be used as a substrate in a chemiluminescent enzyme-linked immunosorbent assay to detect human tissue kininogen. The results showed that the detection sensitivity can reach 1 pM, the calibration curve concentration range is 0.8 pM to 456 pM, and there is no interference from the biological matrix[1].
Compared with other chemiluminescent substrates (CSPD, LUMI-PHOS 530), AMPPD (0.4 mM; 20 min) produces comparable luminescent signal intensities for immunoreactive substances at the same concentration[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 122341-56-4
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性状 Solid
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分子量 382.34
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分子式 C18H23O7P
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Color White to off-white
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SMILES
COC1(C2=CC(OP(O)(O)=O)=CC=C2)C3(C(C4)CC5CC4CC3C5)OO1
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別名
Lumi-Phos Plus; Lumigen PPD; PPD
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Int J Biol Macromol
High-affinity VNARs targeting human hemoglobin: Screening, stability and binding analysis. [Abstract]2025 Apr:299:140290. PMID: 39863199
溶剤 & 溶解度
H2O : 175 mg/mL (457.71 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Legris F, et al. An ultrasensitive chemiluminoenzyme immunoassay for the quantification of human tissue kininogens: application to synovial membrane and cartilage. J Immunol Methods. 1994 Jan 12;168(1):111-21. [Content Brief]
[2]. Green MR, et al. Preparation of Labeled DNA, RNA, and Oligonucleotide Probes. Cold Spring Harb Protoc. 2022 Jan 4;2022(1). [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.6155 mL | 13.0774 mL | 26.1547 mL | 65.3868 mL |
| 5 mM | 0.5231 mL | 2.6155 mL | 5.2309 mL | 13.0774 mL | |
| 10 mM | 0.2615 mL | 1.3077 mL | 2.6155 mL | 6.5387 mL | |
| 15 mM | 0.1744 mL | 0.8718 mL | 1.7436 mL | 4.3591 mL | |
| 20 mM | 0.1308 mL | 0.6539 mL | 1.3077 mL | 3.2693 mL | |
| 25 mM | 0.1046 mL | 0.5231 mL | 1.0462 mL | 2.6155 mL | |
| 30 mM | 0.0872 mL | 0.4359 mL | 0.8718 mL | 2.1796 mL | |
| 40 mM | 0.0654 mL | 0.3269 mL | 0.6539 mL | 1.6347 mL | |
| 50 mM | 0.0523 mL | 0.2615 mL | 0.5231 mL | 1.3077 mL | |
| 60 mM | 0.0436 mL | 0.2180 mL | 0.4359 mL | 1.0898 mL | |
| 80 mM | 0.0327 mL | 0.1635 mL | 0.3269 mL | 0.8173 mL | |
| 100 mM | 0.0262 mL | 0.1308 mL | 0.2615 mL | 0.6539 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
AMPPD 関連分類
HY-15906 関連分類
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