アパミン
Based on 11 publication(s) in Google Scholar
Apamin (Apamine) is an 18 amino acid peptide neurotoxin found in apitoxin (bee venom), is known as a specifically selective blocker of Ca2+-activated K+ (SK) channels and exhibits anti-inflammatory and anti-fibrotic activity.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.96%
- CAS 番号: 24345-16-2
- 分子式: C79H131N31O24S4
- 分子量:2027.34
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 Apamin
More- Nat Commun. 2023 Dec 12;14(1):8255. [Abstract]
- J Headache Pain. 2025 Feb 25;26(1):42. [Abstract]
- Commun Biol. 2026 May 27.
- Life Sci. 2024 Jan 1:336:122326. [Abstract]
- Eur J Pharmacol. 2025 Sep 15:1003:177900. [Abstract]
- Cell Calcium. 2022 Jun;104:102571. [Abstract]
- Heliyon. 2024 Jul 6;10(14):e33994. [Abstract]
- Clin Exp Pharmacol Physiol. 2025 Nov;52(11):e70081. [Abstract]
- Res Sq. 2025 Dec 1:rs.3.rs-7895492. [Abstract]
- Res Sq. 2025 Nov 12.
- bioRxiv. 2025 Jun 6:2025.06.05.657434. [Abstract]
生物活性
K+ channel[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
0.00016 μM
Compound: 14
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Inhibition of Kca2.3 channel expressed in HEK293 cells by thallium flux assay
Inhibition of Kca2.3 channel expressed in HEK293 cells by thallium flux assay
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[PMID: 18774291] |
| HEK293 | IC50 |
0.16 nM
Compound: 14
|
Inhibition of Kca2.3 channel expressed in HEK293 cells by thallium flux assay
Inhibition of Kca2.3 channel expressed in HEK293 cells by thallium flux assay
|
[PMID: 18774291] |
| HEK293 | IC50 |
0.00016 μM
Compound: apamin
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Inhibition of Kca2.3 channel expressed in HEK293 cells by thallium flux assay
Inhibition of Kca2.3 channel expressed in HEK293 cells by thallium flux assay
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[PMID: 18824351] |
| HEK293 | IC50 |
0.16 nM
Compound: apamin
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Inhibition of Kca2.3 channel expressed in HEK293 cells by thallium flux assay
Inhibition of Kca2.3 channel expressed in HEK293 cells by thallium flux assay
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[PMID: 18824351] |
| Hepatocyte | IC50 |
0.001 μM
Compound: 1 Apamin
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Inhibition of apamin-sensitive SKCa channel of guinea-pig hepatocytes
Inhibition of apamin-sensitive SKCa channel of guinea-pig hepatocytes
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[PMID: 15225721] |
Apamin (0.5-2 μg/mL; 24 hours; HSC-T6 cells) treatment markedly reduces the expression of α-SMA in the TGF-β1-induced HSC-T6 cells. Apamin treatment abrogats the activation of p-Smad2/3 and Smad4 induced by TGF-β1[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HSC-T6 cells
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Concentration:0.5 µg/mL, 1 µg/mL and 2 µg/mL
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Incubation Time:24 hours
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Result:Markedly reduced the expression of α-SMA in the TGF-β1-induced HSC-T6 cells. Abrogated the activation of p-Smad2/3 and Smad4 induced by TGF-β1.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:8-week-old C57BL/6 male mice (20-25 g) with DDC feeding[1]
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Dosage:0.1 mg/kg
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Administration:Intraperitoneal injection; twice a week; for 4 weeks
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Result:Resulted in decreased liver injury and proinflammatory cytokine levels. Suppressed the deposition of collagen, proliferation of BECs and expression of fibrogenic genes in the DDC-fed mice.
化学情報
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CAS 番号 24345-16-2
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性状 Solid
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分子量 2027.34
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分子式 C79H131N31O24S4
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Color White to off-white
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別名
Apamin; Apamine
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配列
Cys-Asn-Cys-Lys-Ala-Pro-Glu-Thr-Ala-Leu-Cys-Ala-Arg-Arg-Cys-Gln-Gln-His-NH2 (Disulfide bridge: Cys1-Cys11;Cys3-Cys15)
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シーケンスの短縮
CNCKAPETALCARRCQQH-NH2 (Disulfide bridge: Cys1-Cys11;Cys3-Cys15)
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (11)
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Journal Impact Factor
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Most Recent
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Nat Commun
D-serine reconstitutes synaptic and intrinsic inhibitory control of pyramidal neurons in a neurodevelopmental mouse model for schizophrenia. [Abstract]2023 Dec 12;14(1):8255. PMID: 38086803 -
J Headache Pain
Pregnenolone sulfate potentiates tetrodotoxin-resistant Na+ channels to increase the excitability of dural afferent neurons in rats. [Abstract]2025 Feb 25;26(1):42. PMID: 40000932 -
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Life Sci
Regulatory role of Piezo1 channel in endothelium-dependent hyperpolarization-mediated vasorelaxation of small resistance vessels and its anti-inflammatory action. [Abstract]2024 Jan 1:336:122326. PMID: 38056769 -
Eur J Pharmacol
Novel mechanisms of metformin-induced vasorelaxation of mesenteric arterioles via endothelium-dependent hyperpolarization to treat murine colitis. [Abstract]2025 Sep 15:1003:177900. PMID: 40617384 -
Cell Calcium
Mechanosensitive Piezo1 channel in rat epididymal epithelial cells promotes transepithelial K+ secretion. [Abstract]2022 Jun;104:102571. PMID: 35314382 -
Heliyon
N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine induces endothelium-dependent hyperpolarization-mediated vasorelaxation via store-operated calcium entry mechanism in healthy and intestinal inflammatory mice. [Abstract]2024 Jul 6;10(14):e33994. PMID: 39108891 -
Clin Exp Pharmacol Physiol
Indole-3-Propionic Acid Improves Vascular Function in High-Fat Diet-Induced Obese Mice via eNOS. [Abstract]2025 Nov;52(11):e70081. PMID: 41057979 -
Res Sq
Non-invasive electrical stimulation restores corneal nerve density and function in diabetic neuropathy via KCNN-dependent mechanism. [Abstract]2025 Dec 1:rs.3.rs-7895492. PMID: 41377972 -
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bioRxiv
2025 Jun 6:2025.06.05.657434. PMID: 40502172
溶剤 & 溶解度
H2O : 100 mg/mL (49.33 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (289 KB)
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SDS (420 KB)
- English - EN (420 KB)
- Français - FR (420 KB)
- Deutsch - DE (420 KB)
- Norwegian - NO (420 KB)
- Español - ES (420 KB)
- Swedish - SV (420 KB)
- Italian - IT (420 KB)
- Portuguese - PT (420 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.4933 mL | 2.4663 mL | 4.9326 mL | 12.3314 mL |
| 5 mM | 0.0987 mL | 0.4933 mL | 0.9865 mL | 2.4663 mL | |
| 10 mM | 0.0493 mL | 0.2466 mL | 0.4933 mL | 1.2331 mL | |
| 15 mM | 0.0329 mL | 0.1644 mL | 0.3288 mL | 0.8221 mL | |
| 20 mM | 0.0247 mL | 0.1233 mL | 0.2466 mL | 0.6166 mL | |
| 25 mM | 0.0197 mL | 0.0987 mL | 0.1973 mL | 0.4933 mL | |
| 30 mM | 0.0164 mL | 0.0822 mL | 0.1644 mL | 0.4110 mL | |
| 40 mM | 0.0123 mL | 0.0617 mL | 0.1233 mL | 0.3083 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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