GF 15
GF 15 is an inhibitor of centrosomal clustering during cell mitosis, with an EC50 value of 900 nM for inducing multipolar spindles. GF 15 is a derivative of griseofulvin that inhibits tubulin polymerization at concentrations above 25 μM. GF 15 inhibits tumor growth and significantly prolongs survival in mouse xenograft models of human colon cancer and multiple myeloma.
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- CAS 番号: 888042-13-5
- 分子式: C23H21ClO6
- 分子量:428.86
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Centrosomal clustering[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCC1937 | IC50 |
>10 μM
Compound: 2, GF-15
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Antiproliferative activity against exponentially growing adherent human HCC1937 cells assessed as inhibition of cell proliferation after 72 hrs by luminescence detection based ATPlite assay
Antiproliferative activity against exponentially growing adherent human HCC1937 cells assessed as inhibition of cell proliferation after 72 hrs by luminescence detection based ATPlite assay
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[PMID: 25872984] |
| MDA-MB-231 | IC50 |
1.62 μM
Compound: 2
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Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by celltiter Glo assay
Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 72 hrs by celltiter Glo assay
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[PMID: 27061984] |
| MDA-MB-231 | IC50 |
1.7 μM
Compound: 2
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Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by CellTiter-Glo luminescent assay
Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by CellTiter-Glo luminescent assay
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[PMID: 28258034] |
| MDA-MB-231 | IC50 |
2.1 μM
Compound: 17
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Cytotoxicity against human MDA-MB-231 cells assessed as cell viability after 48 hrs by MTT assay
Cytotoxicity against human MDA-MB-231 cells assessed as cell viability after 48 hrs by MTT assay
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[PMID: 22191585] |
| U2OS | IC50 |
1.3 μM
Compound: 2
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Antiproliferative activity against human U2OS cells after 72 hrs by CellTiter-Glo luminescent assay
Antiproliferative activity against human U2OS cells after 72 hrs by CellTiter-Glo luminescent assay
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[PMID: 28258034] |
| U2OS | IC50 |
1.45 μM
Compound: 2
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Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 72 hrs by celltiter Glo assay
Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 72 hrs by celltiter Glo assay
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[PMID: 27061984] |
GF 15 leads to multipolar mitosis induction with an EC50 of 900 nM. GF 15 exhibits potent cytotoxicity in a concentration-dependent manner against a broad spectrum of tumor cell types including colon, cervix, glioblastoma, pancreas, leukemia, and myeloma-derived cell lines with IC50s of 1-5 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 888042-13-5
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分子量 428.86
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分子式 C23H21ClO6
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SMILES
O=C1[C@]2(C(OCC3=CC=CC=C3)=CC(C[C@H]2C)=O)OC4=C(Cl)C(OC)=CC(OC)=C14
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
Hela cells are treated with GF 15 (0, 0.25, 0.5, 0.75, 1, 1.25 μM) for 24 and/or 48 hours. Cell viability is examined using the MTT assay[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice[1]
Beige-nude Xid mice are inoculated s.c. in the right flank with 3×106 OPM2 or HT29 cells. When a tumor is measurable, mice are assigned to a GF 15 treatment group (20, 100 mg/kg) or the control group for 0-20 days. Tumor burden is measured every alternating day using an electronic caliper.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
GF 15 関連分類
HY-12797 関連分類
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