製品番号: HY-104005

ML335

Name: ML335
Brand: MedChemExpress
SKU: HY-104005
Price: 72.00 USD
Availability: InStock
Rating: 5 (2 reviews)

2 Cited Publications

カスタマーレビュー

Based on 2 publication(s) in Google Scholar

ML335 is a selective activator of both TREK-1 and TREK-2.

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純度 : 99.73%
CAS 番号: 825658-06-8
分子式: C₁₅H₁₄Cl₂N₂O₃S
分子量:373.25

保管条件:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

ML335 構造式

CAS 番号: 825658-06-8

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

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製品説明

ML335 is a selective activator of both TREK-1 and TREK-2.

IC₅₀ & Target

TREK-1, TREK-2

体外実験

Xenopus oocyte two-electrode voltage-clamp measurements show that ML335 and ML402 activate K_2P2.1 and K_2P10.1 but not K_2P4.1 (14.3±2.7 μM, K_2P2.1-ML335; 13.7±7.0 μM, K_2P2.1-ML402; 5.2±0.5 μM, K_2P10.1-ML335; and 5.9±1.6 μM, K_2P10.1-ML402). Swapping the Lys271 equivalent between K_2P2.1 and K_2P4.1 results in a clear phenotype reversal for ML335 and M402 activation. ML335 and ML402 activate K_2P2.1 in HEK293 cells similar to their effects in Xenopus oocytes (5.2±0.8 μM and 5.9±1.6 μM for ML335 and ML402, respectively (n≥3))^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .

化学情報

CAS 番号 825658-06-8
性状 Solid
分子量 373.25
分子式 C₁₅H₁₄Cl₂N₂O₃S
Color White to off-white
SMILES

O=C(NCC1=CC=C(Cl)C=C1Cl)C2=CC=C(NS(=O)(C)=O)C=C2
輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (2)

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J Transl Med

ML335 inhibits TWIK2 channel-mediated potassium efflux and attenuates mitochondrial damage in MSU crystal-induced inflammation. [Abstract]

2024 Aug 22;22(1):785. PMID: 39175013
Sci Rep

Calcium-dependent activation of TREK-1 and TREK-2 background potassium channels by calcineurin. [Abstract]

2025 Dec 29. PMID: 41457157

溶剤 & 溶解度

体外:

DMSO : 155 mg/mL (415.27 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6792 mL

13.3958 mL

26.7917 mL

5 mM

0.5358 mL

2.6792 mL

5.3583 mL

10 mM

0.2679 mL

1.3396 mL

2.6792 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (274 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Lolicato M, et al. K2P2.1 (TREK-1)-activator complexes reveal a cryptic selectivity filter binding site. Nature. 2017 Jul 20;547(7663):364-368. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6792 mL	13.3958 mL	26.7917 mL	66.9792 mL
	5 mM	0.5358 mL	2.6792 mL	5.3583 mL	13.3958 mL
	10 mM	0.2679 mL	1.3396 mL	2.6792 mL	6.6979 mL
	15 mM	0.1786 mL	0.8931 mL	1.7861 mL	4.4653 mL
	20 mM	0.1340 mL	0.6698 mL	1.3396 mL	3.3490 mL
	25 mM	0.1072 mL	0.5358 mL	1.0717 mL	2.6792 mL
	30 mM	0.0893 mL	0.4465 mL	0.8931 mL	2.2326 mL
	40 mM	0.0670 mL	0.3349 mL	0.6698 mL	1.6745 mL
	50 mM	0.0536 mL	0.2679 mL	0.5358 mL	1.3396 mL
	60 mM	0.0447 mL	0.2233 mL	0.4465 mL	1.1163 mL
	80 mM	0.0335 mL	0.1674 mL	0.3349 mL	0.8372 mL
	100 mM	0.0268 mL	0.1340 mL	0.2679 mL	0.6698 mL

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ML335

2 Cited Publications

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ML335

MedChemExpress（MCE）の使用を引用している文献 ML335

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