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ALT-100 (Human IgG4)

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ALT-100 (Human IgG4) is a humanized antibody expressed in CHO, targeting NAMPT. ALT-100 (Human IgG4) consists of a huIgG1/huIgG4 heavy chain and a huκ light chain, with a predicted molecular weight (MW) of 150 kDa. For inflammatory or fibrotic diseases, the isotype control for ALT-100 (Human IgG4) can refer to Human IgG4 (S228P) kappa, Isotype Control (HY-P99003).

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  • 純度: ≥99.0%
  • 分子量:144.6 kDa
  • 保管条件:

    Please store the product under the recommended conditions in the Certificate of Analysis.

  • Experimental Validation Results for ALT-100 (Human IgG4)
  • Experimental Validation Results for ALT-100 (Human IgG4)
  • Experimental Validation Results for ALT-100 (Human IgG4)
  • ALT-100 (Human IgG4) Chemical Structure
    ALT-100 (Human IgG4) 構造式
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Assay Types
  • 1/16

    IgG4-kappa

  • 1/16

    Immobilized Human NAMPT, His Tag can bind ALT-100 (Human IgG4). The EC50 for this effect is 8.465 ng/mL.

  • 1/16

    Flow cytometric analysis of 1X106 MCF-7 cells with ALT-100 (Human IgG4) (HY-P990319, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG4 kappa (HY-P99003, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • ALT-100 (Human IgG4) Chemical Structure
    1/16
  • ALT-100 (Human IgG4)
  • Immobilized Human NAMPT, His Tag can bind ALT-100 (Human IgG4). The EC50 for this effect is 8.465 ng/mL.
  • Flow cytometric analysis of 1X106 MCF-7 cells with ALT-100 (Human IgG4) (HY-P990319, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG4 kappa (HY-P99003, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • IgG4-kappa