country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

製品番号: HY-112765

Copy Link Print Information

AtuFECT01

AtuFECT01 is a cationic lipid component. AtuFECT01 is used to construct AtuPLEX/Atu027 Lipid nanoparticles. AtuFECT01 improves cellular uptake and endosomal escape of siRNA in AtuFECT01-based lipoplexes. Formulation of AtuFECT01 with Tie2 siRNA into lipoplexes reduces Tie2 expression in mouse lung.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号: 869094-19-9
分子式: C₄₃H₉₀Cl₃N₇O₂
分子量:843.58

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

AtuFECT01 構造式

CAS 番号: 869094-19-9

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

体外実験

AtuFECT01-based siRNA-lipoplexes (1-50 nM; 48 h) inhibit PKN3 protein expression in HeLa cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

AtuFECT01-based siRNA-lipoplex (1.88 mg/kg siRNA and 14.5 mg/kg lipid; i.v. via tail vein; once daily; four consecutive days) reduces endothelial CD31 and Tie2 mRNA/protein in lung, heart, and liver and lowers serum soluble Tie2 in healthy nude mice^[2].
DACC/AtuFECT01 lipoplexes (0.75-3 mg siRNA/kg; intravenous bolus; single or once daily; 3 consecutive days) mediate dose-dependent, lung endothelial-specific knockdown of Tie-2, CD31, VEGFR2, VE-Cadherin, and BMPR-2 in C57Bl/6j healthy mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Hsd:NMRI-nu/nu nude (male, 9 weeks)^[2]
Dosage:

1.88 mg/kg siRNA and 14.5 mg/kg lipid
Administration:

i.v. via tail vein; once daily; four consecutive days
Result:

Reduced Tie2 mRNA in lung, heart, and liver after siRNA^Tie2-lipoplex administration.
Reduced CD31 mRNA in lung, heart, and liver after siRNA^CD31-lipoplex administration.
Reduced Tie2 mRNA more prominently than CD31 mRNA in liver.
Inhibited both target genes similarly in lung and heart.
Reduced Tie2 protein in all three analyzed organs after siRNA^Tie2-lipoplex administration.
Reduced CD31 protein in lung and heart after siRNA^CD31-lipoplex administration.
Reduced serum soluble Tie2 on day 5 in siRNA^Tie2-lipoplex-administered mice compared with sucrose, siRNA^PTEN, and siRNA^CD31 control cohorts.
Observed no suppression of endothelial gene expression in kidney and spleen.

Related Protocols :

化学情報

CAS 番号 869094-19-9
分子量 843.58
分子式 C₄₃H₉₀Cl₃N₇O₂
SMILES

N=C(N)NCCC[C@H](N)C(NC[C@H](N)C(N(CCCCCCCCCCCCCCCC)CCCCCCCC/C=C\CCCCCCCC)=O)=O.Cl.Cl.Cl
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Miller AD, et al. Delivery of RNAi therapeutics: work in progress. Expert review of medical devices. 2013 Nov;10(6):781-811. [Content Brief]

[2]. Santel A, et al. A novel siRNA-lipoplex technology for RNA interference in the mouse vascular endothelium. Gene therapy. 2006 Aug;13(16):1222-34.

[3]. Fehring V, et al. Delivery of therapeutic siRNA to the lung endothelium via novel Lipoplex formulation DACC. Molecular therapy : the journal of the American Society of Gene Therapy. 2014 Apr;22(4):811-20.

[4]. Hattori Y, et al. Effect of cationic lipid in cationic liposomes on siRNA delivery into the lung by intravenous injection of cationic lipoplex. Journal of drug targeting. 2019 Feb;27(2):217-227. [Content Brief]

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

お問い合わせ在庫あり

Other size

お問い合わせ