BTC tetrapotassium
BTC tetrapotassium is a cell-impermeant calcium indicator (Kd approximately 7-26 µM) featuring many desirable properties for cellular calcium imaging, including long excitation wavelengths (400/485 nm), low sensitivity to Mg2+, and accuracy of ratiometric measurement.
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- CAS 番号: 216453-54-2
- 分子式: C33H25K4N3O12S
- 分子量:844.02
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
生物活性
製品説明
体外実験
BTC tetrapotassium can be introduced into cells using methods such as patch clamping and microinjection.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
化学情報
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CAS 番号 216453-54-2
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分子量 844.02
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分子式 C33H25K4N3O12S
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SMILES
O=C(O[K])CN(C(C=C(OC1=O)C(C=C1C2=NC3=CC=CC=C3S2)=C4)=C4OCCOC(C=C5C)=C(C=C5)N(CC(O[K])=O)CC(O[K])=O)CC(O[K])=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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Ca2+ Staining Technique
Ca2+ staining is an experimental technique that utilizes specific fluorescent probes (such as Fluo-4 AM, Fura-2, etc.) to qualitatively or quantitatively detect dynamic changes in intracellular Ca2+ concentrations; this is achieved by monitoring the changes in fluorescent signals generated when these probes bind to free intracellular calcium ions. The underlying principle relies primarily on the presence of chelating groups within the probe's molecular structure that possess high affinity for calcium ions.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)