CHAPS hydrate
Based on 4 publication(s) in Google Scholar
CHAPS hydrate is a cholic acid-derived, sulfobetaine-type zwitterionic detergent and micelle-forming agent. CHAPS hydrate exhibits properties of weak cationic or nonionic surfactants in different solution systems, undergoes micellization, and forms small, loose hydrophilic aggregates that are temperature-dependent. CHAPS hydrate stabilizes mononucleosomes under different ionic strengths, reduces nucleosome sequence specificity, promotes sliding of histone cores along DNA, solubilizes Tamm‑Horsfall protein to reduce its interference with urinary exosome isolation, and maintains vesicle structure and the activity of related proteins at the same time. CHAPS hydrate is used to recover native folded fusion proteins, enhance the binding capacity of GST fusion proteins, and restore GST enzyme activity. However, CHAPS hydrate cannot refold proteins denatured by urea, guanidine hydrochloride or heat, nor can it construct the structure of intrinsically disordered proteins. CHAPS hydrate is commonly used in research on the separation and purification of membrane proteins.
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- 純度: 98.65%
- CAS 番号: 331717-45-4
- 分子式: C32H60N2O8S
- 分子量:632.89
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保管条件:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 CHAPS hydrate
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生物活性
CHAPS hydrate forms low aggregation number (5.4-6.7) micelles with cmc values dependent on solvent and temperature (U-shaped cmc vs temperature curve with minimum at ~295 K) and ΔHm0 transitioning from endothermic to exothermic with increasing temperature in aqueous solutions, NaCl solutions, and buffer solutions[1].
CHAPS hydrate (16-4.8 mM; 1-120 h) stabilizes reconstituted mononucleosomes (assembled with human histones H2A, H2B, H3, H4 on 601 sequence DNA) at 0.4 nM concentration at 0°C without altering their structure, with 16 μM preventing dissociation for 1 hour, 1.6 mM maintaining over 50% intact nucleosomes for 120 hours, and 4.8 mM maintaining over 70% intact nucleosomes for 120 hours[2].
CHAPS hydrate (1% (w/v); overnight at 4°C) treatment of pooled urine-derived exosomal pellets (P18, P200) preserves vesicle morphology, exosomal marker distribution, and nephrilysin activity while removing interfering proteins like THP and albumin[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 331717-45-4
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性状 Solid
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分子量 632.89
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分子式 C32H60N2O8S
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Color White to off-white
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SMILES
O[C@H]1[C@@]2([H])[C@@]3([H])[C@@]([C@@](CC3)([H])[C@H](C)CCC(NCCC[N+](C)(C)CCCS(=O)([O-])=O)=O)([C@H](C[C@]2([H])[C@@]4([C@](C[C@@H](CC4)O)([H])C1)C)O)C.O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (4)
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Journal Impact Factor
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Most Recent
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J Colloid Interface Sci
Efficient biofunctionalization of MoS2 nanosheets with peptides as intracellular fluorescent biosensor for sensitive detection of caspase-3 activity. [Abstract]2019 May 1:543:96-105. PMID: 30782521 -
Brain Behav Immun
2026 Feb 24:135:106516. PMID: 41747779 -
Biochem Pharmacol
2025 Apr 12:116941. PMID: 40228633 -
Biochem Pharmacol
2024 Jun 26:226:116392. PMID: 38942091
溶剤 & 溶解度
DMSO : 10 mg/mL (15.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Kroflic A, et al. Thermodynamic characterization of 3-[(3-cholamidopropyl)-dimethylammonium]-1-propanesulfonate (CHAPS) micellization using isothermal titration calorimetry: temperature, salt, and pH dependence. Langmuir. 2012 Jul 17;28(28):10363-71. [Content Brief]
[2]. Menshikova I, et al. Nucleosomes structure and dynamics: effect of CHAPS. Int J Biochem Mol Biol. 2011;2(2):129-137. [Content Brief]
[3]. Musante L, et al. Biochemical and physical characterisation of urinary nanovesicles following CHAPS treatment. PLoS One. 2012;7(7):e37279. [Content Brief]
[4]. Tao H, et al. Purifying natively folded proteins from inclusion bodies using sarkosyl, Triton X-100, and CHAPS. Biotechniques. 2010;48(1):61-64. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5801 mL | 7.9003 mL | 15.8005 mL | 39.5013 mL |
| 5 mM | 0.3160 mL | 1.5801 mL | 3.1601 mL | 7.9003 mL | |
| 10 mM | 0.1580 mL | 0.7900 mL | 1.5801 mL | 3.9501 mL | |
| 15 mM | 0.1053 mL | 0.5267 mL | 1.0534 mL | 2.6334 mL |
CHAPS hydrate 関連分類
HY-15435A 関連分類
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