Elf18
Based on 1 Customer Validation
Elf18 is a peptide fragment of bacterial translation elongation factor Tu (EF-Tu). Elf18 can be recognized by plant pattern recognition receptors, thereby inducing an immune response. Elf18 can enhance plants' resistance to pathogens and can be used in research related to plant immune responses.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.49%
- CAS 番号: 849352-44-9
- 分子式: C91H149N27O28
- 分子量:2069.32
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
生物活性
製品説明
体外実験
Elf18 (300 nM; 30-90 min) can stimulate the production of H2O2 in Arabidopsis cells, but has no such effect in rice cells[1].
Elf18 (5 μM; 0-12 h) induces the expression of ELENA1 in 10-day-old wild-type Arabidopsis seedlings[2].
Elf18 (5 μM; 24 h) affects the expression of PR1 (pathogenesis related-gene 1) and PR2 (pathogenesis related-gene 2) in Arabidopsis plants with ELENA1 knockdown and overexpression[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:ELENA1 KD and OX arabidopsis
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Concentration:5 μM
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Incubation Time:24 h
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Result:Inhibited the expression levels of PR1 and PR2 in ELENA1 KD plants.
Increased the expression levels of PR1 and PR2 in ELENA1 OX plants.
化学情報
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CAS 番号 849352-44-9
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性状 Solid
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分子量 2069.32
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分子式 C91H149N27O28
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Color White to off-white
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配列
Ac-Ser-Lys-Glu-Lys-Phe-Glu-Arg-Thr-Lys-Pro-His-Val-Asn-Val-Gly-Thr-Ile-Gly
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シーケンスの短縮
Ac-SKEKFERTKPHVNVGTIG
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外:
H2O : ≥ 100 mg/mL (48.33 mM)
DMSO : 25 mg/mL (12.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (281 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Furukawa T, et al., Two distinct EF-Tu epitopes induce immune responses in rice and Arabidopsis. Mol Plant Microbe Interact. 2014 Feb;27(2):113-24. [Content Brief]
[2]. Seo JS, et al. ELF18-INDUCED LONG-NONCODING RNA Associates with Mediator to Enhance Expression of Innate Immune Response Genes in Arabidopsis. Plant Cell. 2017 May;29(5):1024-1038. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 0.4833 mL | 2.4163 mL | 4.8325 mL | 12.0813 mL |
| 5 mM | 0.0967 mL | 0.4833 mL | 0.9665 mL | 2.4163 mL | |
| 10 mM | 0.0483 mL | 0.2416 mL | 0.4833 mL | 1.2081 mL | |
| H2O | 15 mM | 0.0322 mL | 0.1611 mL | 0.3222 mL | 0.8054 mL |
| 20 mM | 0.0242 mL | 0.1208 mL | 0.2416 mL | 0.6041 mL | |
| 25 mM | 0.0193 mL | 0.0967 mL | 0.1933 mL | 0.4833 mL | |
| 30 mM | 0.0161 mL | 0.0805 mL | 0.1611 mL | 0.4027 mL | |
| 40 mM | 0.0121 mL | 0.0604 mL | 0.1208 mL | 0.3020 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.