Hericenone A
Hericenone A is an isoindolinone found in the fruiting bodies of Hericium erinaceus. Hericenone A exerts cytotoxic activity against cancer cells. Hericenone A can be used for the research of cancer, such as metastatic human hepatocellular carcinoma.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 126654-52-2
- 分子式: C19H22O5
- 分子量:330.37
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
Hericenone A (compound 6) (5-20 μg/mL; 24 h) dose-dependently inhibits the growth of SMMC-7221 and MHCC-97H human hepatocellular carcinoma cells, with the most potent activity observed at 20 μg/mL for 24 h[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:SMMC-7221, MHCC-97H
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Concentration:5, 10, 20 μg/mL
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Incubation Time:24 h
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Result:Inhibited the growth of SMMC-7221 cells in a dose-dependent manner.
Inhibited the growth of MHCC-97H cells in a dose-dependent manner.
Showed the most potent activity against both cell lines at 20 μg/mL among all tested compounds.
化学情報
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CAS 番号 126654-52-2
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分子量 330.37
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分子式 C19H22O5
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SMILES
OC1=C2C(C(OC2)=O)=CC(OC)=C1C/C=C(C)/CC(/C=C(C)\C)=O
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Structure Classification
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Initial Source
Hericium erinaceus
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Liver Cancer Modeling
Liver cancer can be classified into primary liver cancer and secondary liver cancer. Secondary liver cancer is the metastatic liver cancer. Primary liver cancer includes hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (ICC) and fibrolamellar HCC, of which HCC is the most common form, accounting for approximately 90% of primary liver cancers[1]. HCC mouse models include chemical agent-induced models, transplanted tumor models, and genetic engineered models.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Hericenone A 関連分類
HY-N15388 関連分類
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