Indocyanine green (solution)

At a concentration of 1000 μg/mL, Indocyanine green (Foxgreen) - photodynamic therapy (ICG-PDT) significantly induced the expression of BAX in HGF cells; however, laser irradiation and ICG had no significant effect on the expression of the BCL-2 gene [1].

Guide (The following is the experimental plan we recommend. This plan serves only as a reference guide. The specific operations should be adjusted according to your actual needs.)
1. Preparation of Working Solution^[3] Dilute to 0.03 - 10 μM using Tris buffer or other buffers (optimized according to the experiment).
Note: The working solution should be prepared and used immediately, and stored in a dark place.

2. Organizing the Staining 2.1 The experiment utilized New Zealand white rabbits with Langendorff perfusion for the heart.
2.2 Add Blebbistatin (20 μM) (HY-13813) and Biacetyl monoxime (5 mM) (HY-Y0413) to the perfusion solution to inhibit cardiac contraction while maintaining the electrical activity.
2.3 Gradually increase the concentration of Indocyanine green dye (from 0.03 μM to 10 μM) and add it to the perfusion solution to stain the heart.
2.4 The duration of the light signal recorded by the spectrometer is 3 seconds.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.