ING-1
ING-1 (heMab) is a high-affinity humanized monoclonal antibody against epithelial cell adhesion molecule (Ep-CAM). ING-1 is a transmembrane glycoprotein mediating Ca2+. ING-1 binds to Ep-CAM on tumor cells and exhibits potent in vitro activity, targeting and inhibiting tumor growth and metastasis in mouse cancer models. ING-1 is useful in the research of breast, colorectal, and lung cancers, among other cancers.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.0%
- 分子量:145.77 kDa
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Human IgG1 kappa
Human
EpCAM/TROP1/CD326
ING-1(0.1-10000 nM, 4°C for 5 h) competes similarly with radiolabeled chimeric ING-1 for binding to Ep-CAM in HT -29 human cells, with an Kd of 2 to 5 nM[1].
ING-1(37°C for 4 h) caused a concentration-dependent lysis of 51Cr-labeled BT-20, MCF-7, HT-29, CACO-2, NCI-H1568, PC-3, HPAF-II cells in ADCC and CDC activity assay[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:HT-29 xenograft (3x106/1.5x107) nude mice (20-25 g) model[1][2]
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Dosage:0.1, 0.3, or 1 mg/kg
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Administration:i.v., one/two doses per week, 3 weeks
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Result:Resulted in a dose-dependent reduction in tumor size relative to control, resulted in a 64% reduction in tumor size at the end of the experiment at 1 mg/kg.
Eliminated visible tumor nodules in body cavities of some animals, reduced tumor metastases on lung surfaces, resulted in 86% reduction of the average number of metastases at 1 mg/kg.
Inhibited micrometastases formation in Lung Tissues, reduced the average number of micrometastasis foci by 91% at 1 mg/kg.
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Animal Model:PC-3 xenograft (5x106) nude mice (20-25 g) model[1]
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Dosage:0.1, 0.3, or 1 mg/kg
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Administration:i.v., two doses per week, 3 weeks
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Result:Resulted in a dose-dependent reduction in tumor size relative to control, resulted in a 71% reduction in tumor size at the end of the experiment at 1 mg/kg.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
ELISA, FACS, Functional assay
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Immobilized Human EpCAM Protein, His Tag can bind ING-1. The EC50 for this effect is 29.2 ng/mL.
化学情報
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性状 Liquid
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分子量 145.77 kDa
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Color Colorless to light yellow
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SMILES
N/A
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別名
heMab
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輸送条件
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
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データシート (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
参考文献
[1]. Ammons WS, et al. In vitro and in vivo pharmacology and pharmacokinetics of a human engineered monoclonal antibody to epithelial cell adhesion molecule. Neoplasia. 2003 Mar-Apr;5(2):146-54. [Content Brief]
[2]. Ruan HH, et al. ING-1(heMAb), a monoclonal antibody to epithelial cell adhesion molecule, inhibits tumor metastases in a murine cancer model. Neoplasia. 2003 Nov-Dec;5(6):489-94. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
ING-1 関連分類
HY-P991646 関連分類
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