Naph-EA-mal
Based on 1 publication(s) in Google Scholar
Naph-EA-mal (Thiol-green 1) is a rapid detect and ultrafast turn-on thiol fluorescence probe for protein labeling and bioimaging. Naph-EA-mal (Thiol-green 1) can be used to detect thiols in living cells, label the protein thiols, quantify the concentration of total thiols in cell lysate, and determine the reversible protein thiols oxidation in fixed cells. Ex: 488 nM; Em: 540 nM.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 95.0%
- CAS 番号: 210292-65-2
- 分子式: C22H21N3O4
- 分子量:391.42
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保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 Naph-EA-mal
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生物活性
Imaging thiols in living cells[1]Hep G2 cells incubated with Naph-EA-mal (Thiol-green 1) (1 μM;5 mins), and strong green fluorescence can be observed. When cells are preincubated the cells to block the intracellular thiols with NEM (a thiol block reagent; 100 μM), a weaker fluorescence is observed in cells[1].Monitoring thiol-disulfide levels in fixed cellsDue to the majority of cellular thiols are present in the reduced form, Naph-EA-mal (Thiol-green 1) (10 μM) induces strong fluorescence in Hep G2 cells. After diamide treatment, fluorescent signal sharp dimed is observed[1].The Hep G2 cells, oxidized by diamide (200 μM; 30 min), are fixed by 70% EtOH and the remaining free thiols are blocked by NEM (100 μM). then The cells are further treated with TCEP (5 mM) followed by addition of the Naph-EA-mal (Thiol-green 1) (10 μM). The green fluorescent signal is detected again[1]
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
540
488
化学情報
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CAS 番号 210292-65-2
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性状 Solid
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分子量 391.42
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分子式 C22H21N3O4
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Color Light yellow to orange
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SMILES
O=C1N(CCCC)C(C2=CC=C(NCCN3C(C=CC3=O)=O)C4=CC=CC1=C24)=O
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別名
Thiol-green 1
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Antioxidants (Basel)
2022 Feb 13;11(2):377. PMID: 35204259
溶剤 & 溶解度
DMSO : 33.33 mg/mL (85.15 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5548 mL | 12.7740 mL | 25.5480 mL | 63.8700 mL |
| 5 mM | 0.5110 mL | 2.5548 mL | 5.1096 mL | 12.7740 mL | |
| 10 mM | 0.2555 mL | 1.2774 mL | 2.5548 mL | 6.3870 mL | |
| 15 mM | 0.1703 mL | 0.8516 mL | 1.7032 mL | 4.2580 mL | |
| 20 mM | 0.1277 mL | 0.6387 mL | 1.2774 mL | 3.1935 mL | |
| 25 mM | 0.1022 mL | 0.5110 mL | 1.0219 mL | 2.5548 mL | |
| 30 mM | 0.0852 mL | 0.4258 mL | 0.8516 mL | 2.1290 mL | |
| 40 mM | 0.0639 mL | 0.3194 mL | 0.6387 mL | 1.5968 mL | |
| 50 mM | 0.0511 mL | 0.2555 mL | 0.5110 mL | 1.2774 mL | |
| 60 mM | 0.0426 mL | 0.2129 mL | 0.4258 mL | 1.0645 mL | |
| 80 mM | 0.0319 mL | 0.1597 mL | 0.3194 mL | 0.7984 mL |
Naph-EA-mal 関連分類
HY-D1261 関連分類
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