Phallacidin
Based on 1 Customer Validation
Phallacidin is a natural bicyclic heptapeptide derived from the poisonous mushroom Amanita phalloides. Phallacidin binds to filamentous actin specifically with high affinity, with a Kd of 20 nM. After binding to F-actin, Phallacidin strongly inhibits its depolymerization, stabilizes microfilament structures, and prevents their disruption by drugs such as cytochalasins. When conjugated with a fluorophore, Phallacidin serves as a specific fluorescent probe for F-actin, which is used to clearly visualize the distribution of actin in the cytoskeleton (e.g., stress fibers, cortical peripheral bands) under fluorescence microscopy.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 92.4%
- CAS 番号: 26645-35-2
- 分子式: C37H50N8O13S
- 分子量:846.90
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Pretreatment with phallacidin (0.3 μM; 3-24 h) prevents α-thrombin-induced actin cytoskeletal rearrangements in cultured calf pulmonary artery endothelial monolayers[1].
Pretreatment with phallacidin (0.3 μM; 3-24 h) markedly attenuates α-thrombin-induced increases in 125I-albumin permeability across cultured calf pulmonary artery endothelial monolayers, without altering baseline permeability[1].
Phallacidin specifically labels polymerized F-actin in the kinetochore region of the mitotic spindle in Oedogonium spp. green algal cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 26645-35-2
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性状 Solid
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分子量 846.90
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分子式 C37H50N8O13S
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Color White to off-white
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別名
ファラシジン
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配列
Ala-Trp-Leu-Val-{D-Asp}-Cys-{Hyp} (Sulfide bridge: Trp2- Cys6)
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シーケンスの短縮
AWLV-{D-Asp}-C-{Hyp} (Sulfide bridge: Trp2- Cys6)
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Structure Classification
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Initial Source
Amanita exitialis basidiocarps
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
H2O
Peptide Solubility and Storage Guidelines:
1. Calculate the length of the peptide.
2. Calculate the overall charge of the entire peptide according to the following table:
| Contents | Assign value | |
| Acidic amino acid | Asp (D), Glu (E), and the C-terminal -COOH. | -1 |
| Basic amino acid | Arg (R), Lys (K), His (H), and the N-terminal -NH2 | +1 |
| Neutral amino acid | Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) | 0 |
3. Recommended solution:
| Overall charge of peptide | Details |
| Negative (<0) |
1. Try to dissolve the peptide in water first. 2. If water fails, add NH4OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide. |
| Positive (>0) |
1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO. |
| Zero (=0) |
1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration. |
純度とドキュメンテーション
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データシート (259 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Phillips PG, et al. Phallacidin prevents thrombin-induced increases in endothelial permeability to albumin. Am J Physiol. 1989;257(3 Pt 1):C562-C567. [Content Brief]
[2]. Sampson K, et al. Phallacidin stains the mitotic spindle of the diatom Pinnularia spp. Cell Biol Int. 2004;28(8-9):565-569. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Phallacidin 関連分類
HY-P2031 関連分類
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