SmBiT Tag
Based on 1 Customer Validation
SmBiT Tag is an 11-amino-acid short peptide with extremely low natural affinity for LgBiT, with a Kd value of approximately 190 μM. It serves as a core component of the NanoLuc Binary Technology (NanoBiT) system. NanoBiT is a bioluminescent reporter system based on protein fragment complementation technology, which is used to monitor dynamic protein-protein interactions in live cells and in vitro.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.84%
- CAS 番号: 1628913-44-9
- 分子式: C63H98N14O18
- 分子量:1339.54
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
The SmBiT Tag is used in combination with LgBiT, the other core large fragment component of the NanoBiT system. When SmBiT and LgBiT are fused separately to two interacting target proteins, the binding of these two target proteins brings SmBiT and LgBiT close to each other, promoting their complementary assembly, thereby restoring the high activity of NanoLuc luciferase and generating a detectable bioluminescent signal[1][2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 1628913-44-9
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性状 Solid
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分子量 1339.54
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分子式 C63H98N14O18
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Color White to off-white
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配列
Val-Thr-Gly-Tyr-Arg-Leu-Phe-Glu-Glu-Ile-Leu
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シーケンスの短縮
VTGYRLFEEIL
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
純度とドキュメンテーション
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データシート (258 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Ji Y, et al. Structural basis of peptide recognition and activation of endothelin receptors. Nat Commun. 2023;14(1):1268. Published 2023 Mar 7. [Content Brief]
[2]. Zhang CH, et al. Development of a general bioluminescent activity assay for peptide ligases. FEBS J. 2022;289(17):5241-5258. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
SmBiT Tag 関連分類
HY-P10633 関連分類
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