Thymol blue
Based on 1 Customer Validation
Thymol blue is a reversible pH indicator that responds to the pH of the solution through structural changes of protonation and deprotonation. Thymol blue is red (HI- form) under acidic conditions and blue (I2- form) under alkaline conditions. Quantitative detection is achieved through the absorption peak shift (435 nm/596 nm) of the UV-visible spectrum. Thymol blue selectively responds to pH changes, changing from red to yellow at pH 1.2-2.8 and from yellow to blue at pH 8.0-9.6. Thymol blue can be fixed in a silica gel matrix through sol-gel technology to form a solid-state sensor for in-situ pH measurement in the marine environment and acid-base monitoring in the biomedical field[1][2].
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 76-61-9
- 分子式: C27H30O5S
- 分子量:466.59
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保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
生物活性
Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be modified according to your specific needs).
Thymol blue acid-base indicator protocol (solution state)[1]:
1. Solution preparation
Dissolve Thymol blue sodium salt in ethanol (or water/ethanol mixed solvent) to prepare a 0.1% (w/v) stock solution.
2. pH response test
Take the solution to be tested and add 1-2 drops of Thymol blue indicator:
Acidic conditions (pH<1.7) The solution turns red (HI- form).
Neutral to weakly alkaline (1.7≤pH≤8.9: the solution is yellow (intermediate state.
Strongly alkaline (pH>8.9): the solution is blue (I2- form).
3. Quantitative detection
Use a spectrophotometer to measure the absorbance at 435 nm (HI-) and 596 nm (I2-), calculate the ratio R=A596/A435, and calculate the pH value using the ionization formula.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 76-61-9
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性状 Solid
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分子量 466.59
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分子式 C27H30O5S
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SMILES
O=S1(OC(C2=C(C)C=C(O)C(C(C)C)=C2)(C3=C(C)C=C(O)C(C(C)C)=C3)C4=CC=CC=C41)=O
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別名
Thymolsulphonephthalein
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
純度とドキュメンテーション
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データシート (280 KB)
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取扱説明書 (2659 KB)
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)