Tositumomab
Based on 1 Customer Validation
Tositumomab (Anti-Human CD20 Recombinant Antibody) is a mouse-derived IgG2a monoclonal antibody, targeting to human CD20. Tositumomab can deplete B cells. Tositumomab can be used for the research of cancer, such as lymphoma.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 96.00%
- CAS 番号: 192391-48-3
- 分子量:147.298 kDa
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
生物活性
Mouse IgG2a
Human
MS4A1
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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IgG2a-lambda
ELISA, FACS, Functional assay
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Flow Cytometry analysis of Raji cells labelling CD20/MS4A1 (red) with Tositumomab (anti-CD20/MS4A1) (HY-P99326). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG2a (HY-P99978, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black). -
Loaded Tositumomab on AHC2 biosensor, can bind CD20/MS4A1 Protein, Human (Trx-His, HY-P7896A) with an affinity constant of <1.000E-11 M as determined in BLI assay.
化学情報
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CAS 番号 192391-48-3
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性状 Liquid
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分子量 147.298 kDa
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Color Colorless to light yellow
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SMILES
[Tositumomab]
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別名
Anti-Human CD20 Recombinant Antibody; V10XA53
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輸送条件
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
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データシート (257 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Inhibitory Antibodies User Guide (603 KB)
参考文献
[1]. Havlena GT, et al. Cure of Micrometastatic B-Cell Lymphoma in a SCID Mouse Model Using 213Bi-Anti-CD20 Monoclonal Antibody. J Nucl Med. 2023 Jan;64(1):109-116. [Content Brief]
[2]. Beers SA, et al. Type II (tositumomab) anti-CD20 monoclonal antibody out performs type I (rituximab-like) reagents in B-cell depletion regardless of complement activation. Blood. 2008 Nov 15;112(10):4170-7. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)