TQFL13
TQFL13 is derivative of Thymoquinone (TQ) (HY-D0803) with potent anti-breast cancer activity. TQFL13 exhibits higher cytotoxicity against breast cancer cells (BT549, MDA-MB-231, 4T1). TQFL13 increases apoptosis and blocks the cell cycle at S and G2/G1 phases in breast cancer cells. TQFL13 shows dose-dependent anti-tumor efficacy in mouse breast cancer allograft model. TQFL13 can be used for the study of breast cancer.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 2822560-46-1
- 分子式: C20H23NO5
- 分子量:357.40
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
TQFL13 (0-160 μM, 24 h) exerts cytotoxic activity against BT549, MDA-MB-231, 4T1 breast cancer cells and normal MCF-10A cells, with IC50 values of 23.80 μM, 37.55 μM, 17.59 μM, and > 100 μM, respectively[1].
TQFL13 (48 h) displays anti-viability activity against BT549, MDA-MB-231, 4T1 breast cancer cells and normal MCF-10A cells, with IC50 values of 12.35 μM, 12.14 μM, 12.21 μM, and >100 μM, respectively[1].
TQFL13 (0-20 μM, 24 h) increases apoptosis in 4 T1 cells and blocks the cell cycle at S and G2/G1 phases in BT549 and MDA-MB-231 breast cancer cells[1].
TQFL13 (2.5-5 μM, 0-54 h) inhibits 4 T1 cell growth, migration, and invasion[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:4 T1 and MDA-MB-231 breast cancer cells
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Concentration:0, 5, 10, 20 μM
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Incubation Time:24 h
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Result:Induced the apoptosis.
Reduced the full-length PARP and BCL-2.
Increased the level of BAX.
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Cell Line:BT549 and MDA-MB-231 breast cancer cells
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Concentration:0, 5, 10, 20 μM
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Incubation Time:24 h
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Result:Blocked the cell cycle at S and G2/G1 phases.
Downregulated the level of CyclinB1, CyclinD1and p53.
Upregulated the level of phosphorylated p53 (ser15).
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:4T1 mouse breast cancer cells were injected into the fourth mammary fat pads of female BALB/c mice to construct a breast cancer lung metastasis model [1]
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Dosage:3.75, 7.5, 15 mg/kg
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Administration:i.p., starts 4 days after 4T1 cell inoculation into mammary fat pads, continues until day 30
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Result:Showed dose-dependent anti-tumor efficacy.
Showed no significant changes in body weight.
Altered the morphology of breast tumor cells.
Exerted anti-tumor activity by regulating cell apoptosis and cell cycle signaling pathways.
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Cell Cytotoxicity Assay
- Mammalian live/dead viability and cytotoxicity staining
- Flow cytometric DNA-content cell-cycle staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Apoptosis Solutions
- Protocol for Cell Cycle
- Breast Cancer Modeling
- Cell Viability Determination by MTT Colorimetric Assay
化学情報
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CAS 番号 2822560-46-1
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分子量 357.40
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分子式 C20H23NO5
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SMILES
COC1=CC=C(C(OC)=C1OC)/C=N/C2=C(C(C=C(C2=O)C(C)C)=O)C
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
TQFL13 関連分類
HY-175846 関連分類
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