Thymoquinone

Name: Thymoquinone
Brand: MedChemExpress
SKU: HY-D0803
Rating: 5.0 (14 reviews)

Cat. No.: HY-D0803 Purity: 99.80%: Data Sheet
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Thymoquinone is an orally active natural product isolated from N. sativa Thymoquinone down-regulates the VEGFR2-PI3K-Akt pathway. Thymoquinone has antioxidant, anti-inflammatory, anticancer, antiviral, anticonvulsant, antifungal, antiviral, antiangiogenic activity and hepatoprotective effects. Thymoquinone can be used to study Alzheimer's disease, cancer, cardiovascular disease, infectious disease and inflammation .

For research use only. We do not sell to patients.

CAS No. : 490-91-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

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Customer Review

Based on 14 publication(s) in Google Scholar

Other Forms of Thymoquinone:

Thymoquinone (Standard) Get quote

14 Publications Citing Use of MCE Thymoquinone

Cell Proliferation/Viability Assay

RT-PCR

Histological Imaging/Staining

: Thymoquinone purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2025 Apr;55(4):59. [Abstract]; The viability of H9C2 cells following treatment with varying concentrations of Thymoquinone (TQ) (0, 1, 5, 10 and 20 µM) was assessed.

: Thymoquinone purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2025 Apr;55(4):59. [Abstract]; Images of H9C2 cells stained for wheat germ agglutinin expression treated with Thymoquinone (TQ) (10 μM).

: Thymoquinone purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2025 Apr;55(4):59. [Abstract]; Immunoblotting analysis of ANP and BNP protein expression levels in H9C2 cells treated with Thymoquinone (TQ) (10 μM).

: Thymoquinone purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2025 Apr;55(4):59. [Abstract]; The effects of Thymoquinone (TQ) (10 μM) on collagen I mRNA expression in vivo was assessed using reverse transcription-quantitative PCR.

: Thymoquinone purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2025 Apr;55(4):59. [Abstract]; Masson's trichrome staining for cardiac fibrosis treated with Thymoquinone (TQ) (50 mg/kg. i.g.).

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VEGFR1/Flt-1 VEGFR2/KDR/Flk-1 VEGFR3/Flt-4

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PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

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Akt Akt1 Akt2 Akt3

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

VEGFR2

PI3K

Akt

Cellular Effect

Cell Line	Type	Value	Description	References
518A2	IC₅₀	28 μM Compound: TQ	Cytotoxicity against human 518A2 cells after 72 hrs by MTT assay Cytotoxicity against human 518A2 cells after 72 hrs by MTT assay	[PMID: 21216607]
A2780	IC₅₀	7.9 μM Compound: 1; TQ	Growth inhibition of human A2780 cells after 72 hrs by SRB assay Growth inhibition of human A2780 cells after 72 hrs by SRB assay	[PMID: 29519737]
A2780cis	IC₅₀	7.8 μM Compound: 1; TQ	Growth inhibition of human A2780cis cells after 72 hrs by SRB assay Growth inhibition of human A2780cis cells after 72 hrs by SRB assay	[PMID: 29519737]
CCRF-CEM	EC₅₀	0.3 μM Compound: 3; TQ	Cytotoxicity against human CCRF-CEM cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay Cytotoxicity against human CCRF-CEM cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay	[PMID: 29937978]
CCRF-CEM	EC₅₀	0.3 μM Compound: 6	Cytotoxicity against human CCRF-CEM cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay Cytotoxicity against human CCRF-CEM cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay	[PMID: 29887512]
HCT-116	IC₅₀	50.1 μM Compound: Thymoquinone	Antiproliferative activity against human HCT-116 cells Antiproliferative activity against human HCT-116 cells	[PMID: 31945642]
HEK-293T	CC₅₀	1.4 x 10^-2 μg/mL Compound: Thymoquinone	Cytotoxicity against human 293T cells after 72 hrs by MTT assay Cytotoxicity against human 293T cells after 72 hrs by MTT assay	[PMID: 29100732]
HFF	IC₅₀	33 μM Compound: TQ	Cytotoxicity against human HFF after 72 hrs by MTT assay Cytotoxicity against human HFF after 72 hrs by MTT assay	[PMID: 21216607]
HL-60	IC₅₀	28 μM Compound: TQ	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 21216607]
HT-29	IC₅₀	47 μM Compound: TQ	Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 21216607]
HeLa	IC₅₀	2.06 μM Compound: Thymoquinone	Antiproliferative activity against human HeLa cells assessed as growth inhibition after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25556101]
MCF7	IC₅₀	6.19 μM Compound: Thymoquinone	Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25556101]
OVCAR-8	IC₅₀	11.6 μM Compound: 1; TQ	Growth inhibition of human OVCAR8 cells after 72 hrs by SRB assay Growth inhibition of human OVCAR8 cells after 72 hrs by SRB assay	[PMID: 29519737]
PC-3	IC₅₀	50.1 μM Compound: Thymoquinone	Antiproliferative activity against human PC-3 cells Antiproliferative activity against human PC-3 cells	[PMID: 31945642]
U-937	IC₅₀	101.9 μM Compound: 5	Antiinflammatory activity in PMA-activated human U937 cells assessed as inhibition of LPS-induced TNF-alpha expression preincubated for 2 hrs followed by LPS challenge measured after 6 hrs by ELISA Antiinflammatory activity in PMA-activated human U937 cells assessed as inhibition of LPS-induced TNF-alpha expression preincubated for 2 hrs followed by LPS challenge measured after 6 hrs by ELISA	[PMID: 24491635]

In Vitro

Thymoquinone (20-100 μM, 24-72 h) has anti-proliferation and pro-apoptotic activity in non-small cell lung cancer SCLC cell lines, and has a synergistic effect with Cisplatin (CDDP) (HY-17394) ^[3].
Thymoquinone (100 nM, 24 h) inhibits VEGF expression in HUVECs cells through the VEGFR2/PI3K/Akt signaling pathway^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[3]

Cell Line:	SCLC cell line NCI-H146
Concentration:	20-100 μM
Incubation Time:	24-72 h
Result:	Thymoquinone of 80 and 100 μM showed significant inhibition of cell proliferation, most pronounced at 24 hours. The effect of Thymoquinone alone on cell proliferation diminished over time.

In Vivo

Thymoquinone (5-20 mg/kg/day for 3 days, i.p.) can play a protective role against hepatotoxicity induced by paraquat by activating SOD inhibition mediated by paraquat^[1].
Thymoquinone (10 mg/kg/day for 5 days, p.o.) can protect against Doxorubicin (HY-15142A)-induced cardiotoxicity in rats through superoxide clearance and anti-lipid peroxidation^[2].
Thymoquinone (5-20 mg/kg subcutaneously (s.c.) on Monday, Wednesday and Friday for 3 weeks) alone can effectively reduce the tumor volume of NCI-H460 mouse xenograft model, overcome Cisplatin (CDDP) (HY-17394) resistance, and improve its efficacy ^[3].
Thymoquinone (10 or 20 mg/kg/day for 15 days, i.g.) could clear Aβ plaques and restore neuronal vitality, which could improve the memory ability of Aβ1-42 perfusion rats^[4].
Thymoquinone (single 3 mg/kg, i.p.) down-regulates the VEGFR2-PI3K-Akt pathway and inhibits inflammation and new angiogenesis in asthmatic mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male albino mice (22-27g), Paraquat-induced hepatotoxicity in mice^[1]
Dosage:	5-20 mg/kg/day for 3days
Administration:	i.p.
Result:	Significantly reduced the level of LPO and restored the endogenous antioxidant capacity of liver tissue. Restored SOD activity inhibited by Paraquat. Thymoquinone’s optimum, safe, and protective dose against Paraquat-hepatotoxicity is about 10 mg/kg, which is comparable to the antioxidant properties of Vitamin E.

Clinical Trial

Molecular Weight

164.20

Formula

C₁₀H₁₂O₂

CAS No.

490-91-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C(C)=CC(C(C(C)C)=C1)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (609.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.0901 mL	30.4507 mL	60.9013 mL
5 mM	1.2180 mL	6.0901 mL	12.1803 mL
10 mM	0.6090 mL	3.0451 mL	6.0901 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (30.45 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (30.45 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (282 KB) SDS (393 KB)

COA (251 KB) HNMR (222 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Zeinvand-Lorestani H, et al. Protective role of thymoquinone against paraquat-induced hepatotoxicity in mice. Pestic Biochem Physiol. 2018 Jun;148:16-21. [Content Brief]

[2]. Nagi MN, et al. Protective effect of thymoquinone against doxorubicin-induced cardiotoxicity in rats: a possible mechanism of protection. Pharmacol Res. 2000 Mar;41(3):283-9. [Content Brief]

[3]. Jafri SH, et al. Thymoquinone and cisplatin as a therapeutic combination in lung cancer: In vitro and in vivo. J Exp Clin Cancer Res. 2010 Jul 1;29(1):87. [Content Brief]

[4]. Elibol B, et al. Thymoquinone administration ameliorates Alzheimer's disease-like phenotype by promoting cell survival in the hippocampus of amyloid beta1-42 infused rat model. Phytomedicine. 2020 Dec;79:153324. [Content Brief]

[5]. Su X, et al. Thymoquinone inhibits inflammation, neoangiogenesis and vascular remodeling in asthma mice. Int Immunopharmacol. 2016 Sep;38:70-80. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.0901 mL	30.4507 mL	60.9013 mL	152.2534 mL
	5 mM	1.2180 mL	6.0901 mL	12.1803 mL	30.4507 mL
	10 mM	0.6090 mL	3.0451 mL	6.0901 mL	15.2253 mL
	15 mM	0.4060 mL	2.0300 mL	4.0601 mL	10.1502 mL
	20 mM	0.3045 mL	1.5225 mL	3.0451 mL	7.6127 mL
	25 mM	0.2436 mL	1.2180 mL	2.4361 mL	6.0901 mL
	30 mM	0.2030 mL	1.0150 mL	2.0300 mL	5.0751 mL
	40 mM	0.1523 mL	0.7613 mL	1.5225 mL	3.8063 mL
	50 mM	0.1218 mL	0.6090 mL	1.2180 mL	3.0451 mL
	60 mM	0.1015 mL	0.5075 mL	1.0150 mL	2.5376 mL
	80 mM	0.0761 mL	0.3806 mL	0.7613 mL	1.9032 mL
	100 mM	0.0609 mL	0.3045 mL	0.6090 mL	1.5225 mL

Thymoquinone Related Classifications

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Thymoquinone

Customer Review

Other Forms of Thymoquinone:

14 Publications Citing Use of MCE Thymoquinone

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Thymoquinone

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Thymoquinone Related Antibodies

14 Publications Citing Use of MCE Thymoquinone

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•Related Screening Libraries:

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View All VEGFR Isoform Specific Products:

View All PI3K Isoform Specific Products:

View All Akt Isoform Specific Products:

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Purity & Documentation

References

Customer Review

Thymoquinone Related Antibodies

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Complete Stock Solution Preparation Table

Thymoquinone Related Classifications

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