VHL-SNAP2-5C
Based on 1 Customer Validation
VHL-SNAP2-5C is a PROTAC degrader targeting SNAP-tag fusion proteins, with an IC50 of 0.33 μM for binding to VHL. VHL-SNAP2-5C covalently binds to the SNAP-tag target at one end and recruits the VHL ubiquitin E3 ligase at the other end to form a ternary complex, thereby inducing proteasome-dependent ubiquitination and degradation of SNAP fusion proteins through its heterobifunctional structure. VHL-SNAP2-5C can be used in studies of protein homeostasis and degradation mechanisms.
(Pink: SNAP fusion protein ligand (HY-W128709); Blue: VHL ligand (HY-112078); Black: linker (HY-Y1139)).
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.98%
- 分子式: C42H53ClN8O6S
- 分子量:833.44
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
PROTACs アイソフォーム固有の製品をすべて表示
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生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| THP-1 | IC50 |
>50 μM
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Cytotoxicity against human monocytic THP1 cells measured via Resazurin reagent.
Cytotoxicity against human monocytic THP1 cells measured via Resazurin reagent.
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39444693 |
体外実験
VHL-SNAP2-5C (0.1-100 μM; 24 h) induces target protein degradation in HEK293 SNAP-EGFP cells[1].
VHL-SNAP2-5C (1 μM; 2-8 h) binds to free target proteins and induces their degradation in HEK293 SNAP-EGFP cells[1].
VHL-SNAP2-5C (0.1-5.0 μM; 2-24 h) induces degradation in HAP1 SNAP-CLCaEN cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HAP1 SNAP-CLCaEN
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Concentration:0.1, 0.2, 1.0, 2.0, 5.0 μM
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Incubation Time:2 h, 24 h
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Result:Depleted the target protein nearly below the detection limit at 24 h.
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Cell Line:HAP1 SNAP-CLCaEN
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Concentration:1 μM
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Incubation Time:24 h
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Result:Did not cause bulk structural differences or disassembly in the actin cytoskeleton.
化学情報
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性状 Solid
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分子量 833.44
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分子式 C42H53ClN8O6S
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Color White to off-white
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SMILES
O=C(NCC1=CC=C(COC2=NC(N)=NC(Cl)=C2)C=C1)CCCCCC(N[C@@H](C(C)(C)C)C(N3[C@H](C(N[C@H](C4=CC=C(C5=C(C)N=CS5)C=C4)C)=O)C[C@@H](O)C3)=O)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
体外:
DMSO : 100 mg/mL (119.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
プロトコル
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
純度とドキュメンテーション
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データシート (279 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1998 mL | 5.9992 mL | 11.9985 mL | 29.9962 mL |
| 5 mM | 0.2400 mL | 1.1998 mL | 2.3997 mL | 5.9992 mL | |
| 10 mM | 0.1200 mL | 0.5999 mL | 1.1998 mL | 2.9996 mL | |
| 15 mM | 0.0800 mL | 0.3999 mL | 0.7999 mL | 1.9997 mL | |
| 20 mM | 0.0600 mL | 0.3000 mL | 0.5999 mL | 1.4998 mL | |
| 25 mM | 0.0480 mL | 0.2400 mL | 0.4799 mL | 1.1998 mL | |
| 30 mM | 0.0400 mL | 0.2000 mL | 0.3999 mL | 0.9999 mL | |
| 40 mM | 0.0300 mL | 0.1500 mL | 0.3000 mL | 0.7499 mL | |
| 50 mM | 0.0240 mL | 0.1200 mL | 0.2400 mL | 0.5999 mL | |
| 60 mM | 0.0200 mL | 0.1000 mL | 0.2000 mL | 0.4999 mL | |
| 80 mM | 0.0150 mL | 0.0750 mL | 0.1500 mL | 0.3750 mL | |
| 100 mM | 0.0120 mL | 0.0600 mL | 0.1200 mL | 0.3000 mL |
VHL-SNAP2-5C 関連分類
HY-175417 関連分類
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