X-34
Based on 1 Customer Validation
X-34 is a lipophilic and bright yellow-green fluorescent derivative of Congo red (HY-D0236). X-34 can be used to stain neuritic and diffuse plaques, neurofibrillary tangles (NFTs), neuropil threads, and cerebrovascular amyloid in the brain. X-34 can be used for research of Alzheimer’s disease.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 96.47%
- CAS 番号: 215294-98-7
- 分子式: C24H18O6
- 分子量:402.40
-
保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
生物活性
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Sample preparation[1]
1.1 Paraformaldehyde-fixed tissue was cryoprotected by sequential incubation in 15% and 30% sucrose (in 0.1 M phosphate buffer), frozen, and cryosectioned.
1.2 Frozen sections were collected in Falcon 6-well plates containing
0.1 M phosphate buffer, in a 1:6 series.
1.3 Sections are stored at 4°C until assayed.
1.4 Before staining, the sections are washed for 5 min in PBS.
2. X-34 General Staining Procedure[1]
2.1 Quenched or unquenched tissue sections are taken from PBS.
2.2 Add into a solution of X-34 in 40% ethanol/60% distilled H2O
(adjusted to pH 10 by addition of 1 N NaOH) at the appropriate concentration (100 nM to 1 mM) for the appropriate
period of time (0.5-10 min).
2.3 The sections are then dipped briefly five times in tapwater before differentiation in 0.2% NaOH in 80% ethanol for 2 min.
2.4 The sections are then placed in tapwater for 10 min for microscopic observation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
化学情報
-
CAS 番号 215294-98-7
-
性状 Solid
-
分子量 402.40
-
分子式 C24H18O6
-
Color Light yellow to yellow
-
SMILES
O=C(O)C1=C(O)C=CC(/C=C/C2=CC=C(/C=C/C3=CC(C(O)=O)=C(O)C=C3)C=C2)=C1
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
DMSO : 100 mg/mL (248.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
-
データシート (271 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
取扱説明書 (2659 KB)
参考文献
[1]. Styren SD, et al. X-34, a fluorescent derivative of Congo red: a novel histochemical stain for Alzheimer's disease pathology. J Histochem Cytochem. 2000 Sep;48(9):1223-32. [Content Brief]
[2]. Link CD, et al. Visualization of fibrillar amyloid deposits in living, transgenic Caenorhabditis elegans animals using the sensitive amyloid dye, X-34. Neurobiol Aging. 2001 Mar-Apr;22(2):217-26. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4851 mL | 12.4254 mL | 24.8509 mL | 62.1272 mL |
| 5 mM | 0.4970 mL | 2.4851 mL | 4.9702 mL | 12.4254 mL | |
| 10 mM | 0.2485 mL | 1.2425 mL | 2.4851 mL | 6.2127 mL | |
| 15 mM | 0.1657 mL | 0.8284 mL | 1.6567 mL | 4.1418 mL | |
| 20 mM | 0.1243 mL | 0.6213 mL | 1.2425 mL | 3.1064 mL | |
| 25 mM | 0.0994 mL | 0.4970 mL | 0.9940 mL | 2.4851 mL | |
| 30 mM | 0.0828 mL | 0.4142 mL | 0.8284 mL | 2.0709 mL | |
| 40 mM | 0.0621 mL | 0.3106 mL | 0.6213 mL | 1.5532 mL | |
| 50 mM | 0.0497 mL | 0.2485 mL | 0.4970 mL | 1.2425 mL | |
| 60 mM | 0.0414 mL | 0.2071 mL | 0.4142 mL | 1.0355 mL | |
| 80 mM | 0.0311 mL | 0.1553 mL | 0.3106 mL | 0.7766 mL | |
| 100 mM | 0.0249 mL | 0.1243 mL | 0.2485 mL | 0.6213 mL |
X-34 関連分類
HY-125962 関連分類
Powered by Bioz