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  3. Biochemical Assay Reagents
  4. KB05-Biotin

KB05-Biotin is streptavidin-conjugated KB05 (HY-149461) that retains the site-specific cysteine covalent reactivity of KB05, enabling specific detection of target proteins via the biotin-streptavidin system. KB05-Biotin serves as a site-specific probe in ELISA-ABPP assays for screening electrophilic small molecules that bind to scout fragment-sensitive cysteines on target proteins in cell lysates, without the need for protein purification.

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KB05-Biotin

KB05-Biotin Chemical Structure

CAS No. : 2724919-70-2

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KB05-Biotin Related Antibodies

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Description

KB05-Biotin is streptavidin-conjugated KB05 (HY-149461) that retains the site-specific cysteine covalent reactivity of KB05, enabling specific detection of target proteins via the biotin-streptavidin system. KB05-Biotin serves as a site-specific probe in ELISA-ABPP assays for screening electrophilic small molecules that bind to scout fragment-sensitive cysteines on target proteins in cell lysates, without the need for protein purification[1].

In Vitro

KB05-Biotin (1 μM; 1 h) binds site-specifically to the probe-sensitive cysteine residues: C211 of recombinant wild-type NAGK, C185 of recombinant wild-type HPCAL1, and C516 of recombinant wild-type CDKN2AIP, all expressed in HEK293T cell lysates[1].
KB02-Biotin (1 μM; 1 h) binds site-specifically to the probe-sensitive cysteine C299 in recombinant wild-type AKR1B10 expressed in HEK293T cell lysates[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

KB05-Biotin Related Antibodies
Molecular Weight

913.92

Formula

C42H57BrN8O8S
CAS No.
SMILES

O=C(NCCOCCOCCOCCOCCN1N=NC(CCCCNC(C2=CC=C(N(C(C=C)=O)C3=CC=C(Br)C=C3)C=C2)=O)=C1)CCCC[C@H]4[C@]5([H])[C@](NC(N5)=O)([H])CS4
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (109.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0942 mL 5.4709 mL 10.9419 mL
5 mM 0.2188 mL 1.0942 mL 2.1884 mL
View the Complete Stock Solution Preparation Table

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Purity & Documentation
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Complete Stock Solution Preparation Table

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.0942 mL 5.4709 mL 10.9419 mL 27.3547 mL
5 mM 0.2188 mL 1.0942 mL 2.1884 mL 5.4709 mL
10 mM 0.1094 mL 0.5471 mL 1.0942 mL 2.7355 mL
15 mM 0.0729 mL 0.3647 mL 0.7295 mL 1.8236 mL
20 mM 0.0547 mL 0.2735 mL 0.5471 mL 1.3677 mL
25 mM 0.0438 mL 0.2188 mL 0.4377 mL 1.0942 mL
30 mM 0.0365 mL 0.1824 mL 0.3647 mL 0.9118 mL
40 mM 0.0274 mL 0.1368 mL 0.2735 mL 0.6839 mL
50 mM 0.0219 mL 0.1094 mL 0.2188 mL 0.5471 mL
60 mM 0.0182 mL 0.0912 mL 0.1824 mL 0.4559 mL
80 mM 0.0137 mL 0.0684 mL 0.1368 mL 0.3419 mL
100 mM 0.0109 mL 0.0547 mL 0.1094 mL 0.2735 mL
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KB05-Biotin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

KB05-Biotin2724919-70-2Biochemical Assay ReagentsCDKN2AIPSLC4A11FAM40Anonsmall cell lung cancersHPCAL1NAGKAKR1B10HEK293T cell lysatesSMARCD1ALG3Inhibitorinhibitorinhibit

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