KB05yne

Cat. No.: HY-177554 Purity: 99.97%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

KB05yne is a site-selective, alkyne-functionalized electrophilic "probe" fragment probe designed for covalent ligand discovery, with preferential reactivity toward specific cysteine residues in proteins. KB05yne strongly labels wild-type target proteins (e.g., CDKN2AIP, HPCAL1) but does not label their cysteine-to-alanine mutants, confirming site-specific cysteine reactivity. KB05yne can be used for the discovery and optimization of site-specific covalent ligands, and is applicable to proteins with diverse structures and functions, especially in studies targeting cysteine residues in cancer-related or difficult-to-purify proteins.

For research use only. We do not sell to patients.

KB05yne Chemical Structure

CAS No. : 2724919-69-9

Size	Stock	Quantity
Oil + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Oil
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Description

In Vitro

KB05yne exhibits preferential reactivity toward specific cysteine residues in proteins, and strongly labels wild-type target proteins (e.g., CDKN2AIP, HPCAL1)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

425.32

Formula

C₂₂H₂₁BrN₂O₂

CAS No.

2724919-69-9

Appearance

Oil

Color

Colorless to light yellow

SMILES

O=C(NCCCCC#C)C1=CC=C(N(C2=CC=C(Br)C=C2)C(C=C)=O)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (235.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3512 mL	11.7559 mL	23.5117 mL
5 mM	0.4702 mL	2.3512 mL	4.7023 mL
10 mM	0.2351 mL	1.1756 mL	2.3512 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (269 KB) SDS (252 KB)

COA (246 KB) HNMR (267 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

References

[1]. Crowley VM, et al. Functionalized Scout Fragments for Site-Specific Covalent Ligand Discovery and Optimization. ACS Cent Sci. 2021;7(4):613-623. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3512 mL	11.7559 mL	23.5117 mL	58.7793 mL
	5 mM	0.4702 mL	2.3512 mL	4.7023 mL	11.7559 mL
	10 mM	0.2351 mL	1.1756 mL	2.3512 mL	5.8779 mL
	15 mM	0.1567 mL	0.7837 mL	1.5674 mL	3.9186 mL
	20 mM	0.1176 mL	0.5878 mL	1.1756 mL	2.9390 mL
	25 mM	0.0940 mL	0.4702 mL	0.9405 mL	2.3512 mL
	30 mM	0.0784 mL	0.3919 mL	0.7837 mL	1.9593 mL
	40 mM	0.0588 mL	0.2939 mL	0.5878 mL	1.4695 mL
	50 mM	0.0470 mL	0.2351 mL	0.4702 mL	1.1756 mL
	60 mM	0.0392 mL	0.1959 mL	0.3919 mL	0.9797 mL
	80 mM	0.0294 mL	0.1469 mL	0.2939 mL	0.7347 mL
	100 mM	0.0235 mL	0.1176 mL	0.2351 mL	0.5878 mL

KB05yne Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

KB05yne2724919-69-9Biochemical Assay ReagentsSMARCD1RamosNAGKHEK293TH460HPCAL1FAM40ASLC4A11ALG3CDKN2AIPInhibitorinhibitorinhibit

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KB05yne

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