KCC2 Modulator-1
KCC2 Modulator-1 (example 52) is a KCC2 modulator. KCC2 Modulator-1 is used for the research of neurological disorders.
For research use only. We do not sell to patients.
- CAS No.: 2704531-36-0
- Formula: C35H41N5O3
- Molecular Weight:579.73
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
C50: 0.146 μM (KCC2)[1].
KCC2 is the only specific Cl- efflux transporter in mature neurons, which determines the inhibitory function of GABA; decreased expression/activity leads to neuronal hyperexcitability, making it a common core target for epilepsy, neuropathic pain and developmental encephalopathy.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 2704531-36-0
-
Molecular Weight 579.73
-
Formula C35H41N5O3
-
SMILES
O=C1C2=C(CN1C(C)C)C(NC3=CC=C(C4=CC=C(OCCCCCC#C)C=C4)C=C3)=NC(N5CCOC(C6CC6)C5)=N2
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
DMSO : 100 mg/mL (172.49 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (8.62 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7249 mL | 8.6247 mL | 17.2494 mL | 43.1235 mL |
| 5 mM | 0.3450 mL | 1.7249 mL | 3.4499 mL | 8.6247 mL | |
| 10 mM | 0.1725 mL | 0.8625 mL | 1.7249 mL | 4.3124 mL | |
| 15 mM | 0.1150 mL | 0.5750 mL | 1.1500 mL | 2.8749 mL | |
| 20 mM | 0.0862 mL | 0.4312 mL | 0.8625 mL | 2.1562 mL | |
| 25 mM | 0.0690 mL | 0.3450 mL | 0.6900 mL | 1.7249 mL | |
| 30 mM | 0.0575 mL | 0.2875 mL | 0.5750 mL | 1.4375 mL | |
| 40 mM | 0.0431 mL | 0.2156 mL | 0.4312 mL | 1.0781 mL | |
| 50 mM | 0.0345 mL | 0.1725 mL | 0.3450 mL | 0.8625 mL | |
| 60 mM | 0.0287 mL | 0.1437 mL | 0.2875 mL | 0.7187 mL | |
| 80 mM | 0.0216 mL | 0.1078 mL | 0.2156 mL | 0.5390 mL | |
| 100 mM | 0.0172 mL | 0.0862 mL | 0.1725 mL | 0.4312 mL |
KCC2 Modulator-1 Related Classifications
HY-160081 Related Classifications
Powered by Bioz