5-IAF
Based on 1 publication(s) in Google Scholar
5-IAF (5-Iodoacetamidofluorescein) is an idoacetamide derivate of fluoresceine. 5-IAF can be used as fluorescent probe that labels proteins and other molecules having free thiols (cysteine side chains).
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 63368-54-7
- Formula: C22H14INO6
- Molecular Weight:515.25
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 5-IAF
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Biological Activity
8 μM GSH sample solution is derivatized with 8, 80, 200, 400, 800, 1600, 2000, 2500 μM 5-IAF, respectively and analyzed with CE-LIF. The highest signal intensity is obtained with 400 and 800 μM 5-IAF[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
515
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Chemical Information
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CAS No. 63368-54-7
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Appearance Solid
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Molecular Weight 515.25
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Formula C22H14INO6
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Color Light yellow to orange
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SMILES
O=C(NC1=CC2=C(C3(C4=C(OC5=C3C=CC(O)=C5)C=C(O)C=C4)OC2=O)C=C1)CI
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Synonyms
5-Iodoacetamidofluorescein
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Redox Biol
Endogenous metabolite N-chlorotaurine attenuates antiviral responses by facilitating IRF3 oxidation. [Abstract]2025 Mar:80:103492. PMID: 39799640
Solvent & Solubility
DMSO : 50 mg/mL (97.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
HepG2 cells are lysed in 1 mL 2% acetonitrile in water, and transfered to a 1.5 mL centrifuge tube. An additional 200 µL 2% acetonitrile is used to rinse the dish and combined with the cell extract. To measure the free GSH concentration, three aliquots of 80 µL cell extract are immediately transferred to 0.5 mL centrifuge tubes after a quick mix. Then 20 µL 50 µM NAC is added, followed by the addition of 100 µL 400 µM 5-IAF to the mixture. The derivatization is conducted at room temperature in dark for 1 h, after which the mixtures are centrifuged at 9000× g for 5 min to sediment the insoluble proteins. 10 µL supernatant is diluted 100× with water and analyzed immediately by CE-LIF[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Salvatore Sotgia, et al. Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein (5-IAF) and Fluorescence Detection. PLoS One. 2013; 8(7): e70374. [Content Brief]
[2]. Yan Wang, et al. Determination of free and protein-bound glutathione in HepG2 cells using capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr A. 2009 Apr 17;1216(16):3533-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9408 mL | 9.7040 mL | 19.4081 mL | 48.5201 mL |
| 5 mM | 0.3882 mL | 1.9408 mL | 3.8816 mL | 9.7040 mL | |
| 10 mM | 0.1941 mL | 0.9704 mL | 1.9408 mL | 4.8520 mL | |
| 15 mM | 0.1294 mL | 0.6469 mL | 1.2939 mL | 3.2347 mL | |
| 20 mM | 0.0970 mL | 0.4852 mL | 0.9704 mL | 2.4260 mL | |
| 25 mM | 0.0776 mL | 0.3882 mL | 0.7763 mL | 1.9408 mL | |
| 30 mM | 0.0647 mL | 0.3235 mL | 0.6469 mL | 1.6173 mL | |
| 40 mM | 0.0485 mL | 0.2426 mL | 0.4852 mL | 1.2130 mL | |
| 50 mM | 0.0388 mL | 0.1941 mL | 0.3882 mL | 0.9704 mL | |
| 60 mM | 0.0323 mL | 0.1617 mL | 0.3235 mL | 0.8087 mL | |
| 80 mM | 0.0243 mL | 0.1213 mL | 0.2426 mL | 0.6065 mL |
5-IAF Related Classifications
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