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Cat. No.: HY-173189A

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2-5A pentasodium solution (100 mM)

(Synonyms: 2′,5′-ApApA pentasodium; 2′,5′-trioligoadenylate pentasodium; 5'-O-Triphosphoryladenylyl-(2'→5')-adenylyl-(2'→5')-adenosine pentasodium)

고객리뷰

Based on 1 Customer Validation

2-5A pentasodium solution (100 mM) (2′,5′-ApApA pentasodium solution (100 mM)) is a 5'-triphosphorylated (2',5') oligoadenylate. When covalently linked to an antisense molecule complementary to the target sequence, 2-5A pentasodium solution (100 mM) activates RNase L, which subsequently degrades the target RNA bound to the 2-5A pentasodium solution (100 mM) chimera. 2-5A pentasodium solution (100 mM) can be used in research related to respiratory syncytial virus infection, colorectal adenocarcinoma and melanoma.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity: 90.0%
화학식: C₃₀H₃₅N₁₅Na₅O₂₅P₅
분자량:1275.50

보관:

Solution, -20°C, 2 years

2-5A pentasodium solution (100 mM) Chemical Structure

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
순도&문서
References
Calculators
Help & FAQs
Classifications
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Biological Activity

제품 설명

IC₅₀ & Target

RNASE L

All DNA/RNA Synthesis Isoforms :

In Vitro

2-5A (1 μM; 4 h) pentasodium solution (100 mM) is imported by human HEK293T cells and BJ1 fibroblasts to activate RNase L-dependent IFN/ISG induction, while mouse MEFs, MC38 cells, BMDMs, human A549 cells, and THP-1 monocytes do not exhibit robust 2-5A pentasodium solution (100 mM) import activity^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[3]

Cell Line:

human HEK293T cells, human BJ1 fibroblasts, mouse MEFs, mouse MC38 cells, mouse bone-marrow-derived macrophages (BMDMs), human A549 cells, human THP-1 monocytes
Concentration:

1 μM
Incubation Time:

4 h
Result:

Stimulated significant IFNB1 and CXCL10 mRNA expression in human HEK293T cells and BJ1 fibroblasts.
Abolished IFN/ISG response in RNase L-knockout HEK293T and BJ1 cells.
Failed to induce robust IFN/ISG response in mouse MEFs, MC38 cells, BMDMs, human A549 cells, and THP-1 monocytes.
Induced robust IFN/ISG expression in all tested cell lines when transfected with Lipofectamine.

In Vivo

2-5A (10 μg; peritumoral injection; once daily; 3 consecutive days) pentasodium solution (100 mM) complexed with lipofectamine significantly inhibits MC38 colorectal carcinoma tumor growth in wild-type C57BL/6J mice^[3].
2-5A (10 μg; peritumoral injection; once daily; 3 consecutive days) pentasodium solution (100 mM) complexed with lipofectamine significantly inhibits B16 melanoma tumor growth in wild-type C57BL/6J mice^[3].
2-5A (10 μg; peritumoral injection; once daily; 3 consecutive days) pentasodium solution (100 mM) complexed with lipofectamine significantly inhibits sgRnasel MC38 colorectal carcinoma tumor growth in wild-type C57BL/6J mice^[3].
2-5A (10 μg; peritumoral injection; once daily; 3 consecutive days) pentasodium solution (100 mM) complexed with lipofectamine does not inhibit MC38 colorectal carcinoma tumor growth in RnaseI^−/− mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6J wild-type (6-8 weeks old, same sex, subcutaneous implantation of 5×10⁵ WT MC38 cells)^[3]
Dosage:

10 μg (complexed with lipofectamine)
Administration:

peritumoral injection; once daily; 3 consecutive days
Result:

Significantly inhibited MC38 tumor growth compared to PBS, naked 2-5A (TEA), or lipofectamine alone.
Showed statistically significant difference in tumor volume between the lipofectamine+2-5A group and lipofectamine alone group (P=0.0142).

Animal Model:

C57BL/6J wild-type (6-8 weeks old, same sex, subcutaneous implantation of 5×10⁵ B16 cells)^[3]
Dosage:

10 μg (complexed with lipofectamine)
Administration:

peritumoral injection; once daily; 3 consecutive days
Result:

Significantly inhibited B16 melanoma tumor growth compared to lipofectamine alone (P=0.0185).

Chemical Information

Appearance Liquid
분자량 1275.50
화학식 C₃₀H₃₅N₁₅Na₅O₂₅P₅
Color Colorless to light yellow
SMILES

NC1=NC=NC2=C1N=CN2[C@H]3[C@H](OP(OC[C@@H](O[C@H]([C@@H]4OP(OC[C@@H](O[C@H]([C@@H]5O)N6C=NC7=C6N=CN=C7N)[C@H]5O)(O[Na])=O)N8C=NC9=C8N=CN=C9N)[C@H]4O)(O[Na])=O)[C@H](O)[C@@H](COP(OP(OP(O)(O[Na])=O)(O[Na])=O)(O[Na])=O)O3
Synonyms

2′,5′-ApApA pentasodium; 2′,5′-trioligoadenylate pentasodium; 5'-O-Triphosphoryladenylyl-(2'→5')-adenylyl-(2'→5')-adenosine pentasodium
선적

Room temperature in continental US; may vary elsewhere.
보관

Solution, -20°C, 2 years

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (284 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Barnard DL, et al. 2-5A-DNA conjugate inhibition of respiratory syncytial virus replication: effects of oligonucleotide structure modifications and RNA target site selection. Antiviral research. 1999 Apr;41(3):119-34. [Content Brief]

[2]. Wang S, et al. 2-5A is an immunotransmitter that fuels RNase L immunity. Immunity. 2025 Apr 08;58(4):770-772. [Content Brief]

[3]. Huai W, et al. OAS cross-activates RNase L intercellularly through cell-to-cell transfer of 2-5A to spread innate immunity. Immunity. 2025 Apr 08;58(4):797-810.e6. [Content Brief]

[4]. Kalinichenko EN, et al. 3'-Fluoro-3'-deoxy analogs of 2-5A 5'-monophosphate: binding to 2-5A-dependent endoribonuclease and susceptibility to (2'-5')phosphodiesterase degradation. Biochemical and biophysical research communications. 1990 Feb 28;167(1):20-6. [Content Brief]

Calculators

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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2-5A pentasodium solution (100 mM)

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Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
순도&문서
References

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2-5A pentasodium solution (100 mM)

All DNA/RNA Synthesis Isoforms

Biological Activity

Chemical Information

순도&문서

References

Calculators

Help & FAQs

2-5A pentasodium solution (100 mM) Related Classifications

HY-173189A Related Classifications

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