CFSE-Tz

CFSE-Tz (compound 1) (1 μM; 1 hour, up to 80 minutes) and its intracellular product mimic CFSE-Tz-amide (compound 17) exhibit a dose-independent fluorescence turn-on effect after esterase hydrolysis and an IEDDA reaction with strained alkene-modified nutrient analogs; among these, the nutrient modified with trans-cyclooctene shows the fastest reaction rate^[1].
CFSE-Tz exhibits no toxicity toward HeLa cells^[1].
CFSE-Tz (0-50 μM; 30 minutes, 15-120 minutes) enables concentration- and time-dependent detection of energy-dependent nutrient uptake in HeLa cells^[1].
CFSE-Tz (50 μM; 30 minutes, up to 80 minutes) enables real-time live-cell monitoring of nutrient uptake in HeLa cells, and the intracellular IEDDA reaction achieves a completion rate of >80% within 15 minutes of nutrient uptake^[1].
CFSE-Tz (50 μM; 30 minutes) enables live-cell, single-cell visualization of nutrient uptake in HeLa cells via confocal microscopy, with the fluorescent signal increasing over time as nutrient uptake and intracellular IEDDA reactions occur^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.