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Cat. No.: HY-D1713

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CM-H2DCFDA

7 Cited Publications

고객리뷰

Based on 7 publication(s) in Google Scholar

CM-H2DCFDA is a derivative of H2DCFDA (HY-D0940). CM-H2DCFDA can be used to determine cellular oxidant levels (Ex/Em: 495/530 nm). CM-H2DCFDA is light-sensitive.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity: 91%
CAS No.: 850013-49-9
화학식: C₂₇H₁₉Cl₃O₈
분자량:577.79

보관:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

CM-H2DCFDA Chemical Structure

CAS No.: 850013-49-9

사이즈

가격

재고

수량

Amount: USD 0.00

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- In Vitro
Chemical Information
Publications (7)
용액&용해도
- In Vitro
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

CM-H2DCFDA is a derivative of H2DCFDA (HY-D0940). CM-H2DCFDA can be used to determine cellular oxidant levels (Ex/Em: 495/530 nm). CM-H2DCFDA is light-sensitive^[1].

In Vitro

Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
1. Prepare the storage solution and working solution 1.1 Prepare a 5 mM stock solution of CM-H2DCFDA using anhydrous DMSO.
1.2 Dilute the stored solution with pre-warmed serum-free cell culture medium or PBS to prepare a working solution of 1-10 μM CM-H2DCFDA.
Note: Please adjust the concentration of the CM-H2DCFDA working solution according to the actual situation, and prepare it as needed.
2. Cell Staining (Suspension Cells) 2.1 Centrifuge the cells and add PBS for two washes, each for 5 minutes. The cell density is 1×10⁶ cells/mL.
2.2 Add 1 mL of the dye working solution and incubate at room temperature for 5 to 30 minutes.
2.3 400 grams, centrifuge for 3-4 minutes, then discard the supernatant.
2.4 Add PBS to wash the cells twice, for 5 minutes each time.
2.5 After resuspending the cells in 1 mL of serum-free medium or PBS, observe them using a fluorescence microscope or a flow cytometer.
3. Cell Staining (Monolayer Cells) 3.1 Cultivate adherent cells on sterile coverslips.
3.2 Remove the cover glass from the culture medium and aspirate away the excess medium.
3.3 Add 100 μL of the dye working solution and gently shake to ensure complete coverage of the cells. Incubate for 5 to 30 minutes.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 850013-49-9
Appearance Solid
분자량 577.79
화학식 C₂₇H₁₉Cl₃O₈
Color White to off-white
SMILES

CC(OC1=C(Cl)C=C2C(C3=C(C=CC(CCl)=C3)C(OC(C)=O)=O)C4=CC(Cl)=C(OC(C)=O)C=C4OC2=C1)=O.CC(OC5=C(Cl)C=C6C(C7=C(C=C(CCl)C=C7)C(OC(C)=O)=O)C8=CC(Cl)=C(OC(C)=O)C=C8OC6=C5)=O
선적

Room temperature in continental US; may vary elsewhere.
보관

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (7)

All OthersCell Imaging/Staining

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Journal Impact Factor

J Hazard Mater

Species-specific impacts of fibrous microplastics on behavior, survival and oxidative stress in freshwater zooplankton. [Abstract]

2025 Dec 5:501:140718. PMID: 41371136

CM-H2DCFDA purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Dec 5:501:140718. [Abstract]

CM-H2DCFDA (9.62 µM). ROS levels in T. platyurus and B. calyciflorus after 24-h of exposure to fibrous MPs.
J Transl Med

CAV3 alleviates diabetic cardiomyopathy via inhibiting NDUFA10-mediated mitochondrial dysfunction. [Abstract]

2024 Apr 26;22(1):390. PMID: 38671439

CM-H2DCFDA purchased from MedChemExpress. Usage Cited in: J Transl Med. 2024 Apr 26;22(1):390. [Abstract]

Representative images of DHE staining and ROS (CM-H2DCFDA) level quantification analysis of the hearts of db/db mice transfected or untransfected with AAV-CAV3 or AAV-shNDUFA10.
Cell Rep

The m5C methyltransferase NSUN2 promotes progression of acute myeloid leukemia by regulating serine metabolism. [Abstract]

2025 May 7;44(5):115661. PMID: 40343793
J Cell Commun Signal

Moscatilin alleviates oxidative stress and inflammatory response of Müller cells in diabetic retinopathy through suppressing the p38 mitogen-activated protein kinase/c-Jun N-terminal kinase and nuclear factor kappa-B signaling pathways. [Abstract]

2025 Feb 19;19(1):e12059. PMID: 39975983

CM-H2DCFDA purchased from MedChemExpress. Usage Cited in: J Cell Commun Signal. 2025 Feb 19;19(1):e12059. [Abstract]

Measurement of intracellular reactive oxygen species production by using the oxidation‐sensitive fluoroprobe CM‐H2DCFDA (10 μM) as a substrate.
Mol Med Rep

Obacunone inhibits ferroptosis through regulation of Nrf2 homeostasis to treat diabetic nephropathy. [Abstract]

2025 May;31(5):135. PMID: 40116089

CM-H2DCFDA purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2025 May;31(5):135. [Abstract]

Levels of ROS (10 µM CM-H2DCFDA) in HK-2 cells from each group were measured using immunofluorescence.
Front Public Health

Effects of ozone exposure on lipid metabolism in Huh-7 human hepatoma cells. [Abstract]

2023 Jul 13:11:1222762. PMID: 37521985
PLoS One

Therapeutic potential of salidroside in type I diabetic erectile dysfunction: Attenuation of oxidative stress and apoptosis via the Nrf2/HO-1 pathway. [Abstract]

2024 Jul 11;19(7):e0306926. PMID: 38990890

용액&용해도

In Vitro:

DMSO : 3 mg/mL (5.19 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7307 mL

8.6537 mL

17.3073 mL

5 mM

0.3461 mL

1.7307 mL

3.4615 mL

10 mM

---

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Oparka M, et al. Quantifying ROS levels using CM-H2DCFDA and HyPer. Methods. 2016 Oct 15;109:3-11. [Content Brief]

[2]. Kolarova H, et al. Production of reactive oxygen species after photodynamic therapy by porphyrin sensitizers. Gen Physiol Biophys. 2008 Jun;27(2):101-5. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.7307 mL	8.6537 mL	17.3073 mL	43.2683 mL
DMSO	5 mM	0.3461 mL	1.7307 mL	3.4615 mL	8.6537 mL

Keywords

CM-H2DCFDA

7 Cited Publications

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Minimum order quantity

100 mg

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Amount: USD 0.00

Biological Activity
- 제품 설명
- In Vitro
Chemical Information
Publications (7)
용액&용해도
- In Vitro
순도&문서
References

1/16
1/16

CM-H2DCFDA purchased from MedChemExpress. Usage Cited in: J Hazard Mater. 2025 Dec 5:501:140718. [Abstract]

Customer Validation
CM-H2DCFDA (9.62 µM). ROS levels in T. platyurus and B. calyciflorus after 24-h of exposure to fibrous MPs.
1/16

CM-H2DCFDA purchased from MedChemExpress. Usage Cited in: J Cell Commun Signal. 2025 Feb 19;19(1):e12059. [Abstract]

Customer Validation
Measurement of intracellular reactive oxygen species production by using the oxidation‐sensitive fluoroprobe CM‐H2DCFDA (10 μM) as a substrate.
1/16

CM-H2DCFDA purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2025 May;31(5):135. [Abstract]

Customer Validation
Levels of ROS (10 µM CM-H2DCFDA) in HK-2 cells from each group were measured using immunofluorescence.
1/16

CM-H2DCFDA purchased from MedChemExpress. Usage Cited in: J Transl Med. 2024 Apr 26;22(1):390. [Abstract]

Customer Validation
Representative images of DHE staining and ROS (CM-H2DCFDA) level quantification analysis of the hearts of db/db mice transfected or untransfected with AAV-CAV3 or AAV-shNDUFA10.

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CM-H2DCFDA

Publications Citing Use of MedChemExpress (MCE) CM-H2DCFDA

Biological Activity

Chemical Information

Publications (7)

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

CM-H2DCFDA Related Classifications

HY-D1713 Related Classifications