CO probe 1

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
CO probe 1 CO imaging steps in living cells^[1]:
1. Before the cell imaging experiment, cells were seeded in 24-well culture plates overnight.
2. Set up control group and fluorescence group. Control group: Living cells were incubated with CO probe 1 (1 μM) at 37 °C for 30 minutes. Fluorescence group: Living cells were incubated with a mixture of CO probe 1 and PdCl₂ (1 μM each) at 37 °C for 30 minutes.
3. After the above two groups were incubated, they were washed three times with PBS and then imaged.
* For monitoring exogenous CO, HeLa cells were pretreated with CORM-3 (HY-100581) (1, 5, and 10 μM, respectively) at 37 °C for 30 min and then incubated with a mixture of CO probe 1 and PdCl₂ (1 μM each) at 37 °C for 30 min. Cells were washed with PBS buffer before imaging.
* For monitoring CO stimulated by heme, the procedure was essentially the same as for imaging exogenous CO, but heme (100 μM) was used instead of CORM 3 (HY-100581) in the cell pretreatment.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.