Formycin triphosphate

Formycin triphosphate is not reduced in the cell-free Lactobacillus leichmannii ribonucleotide reductase assay in the absence of dGTP, but acts as a linear competitive inhibitor of ATP, GTP, and UTP reduction with Ki values of 0.93, 0.67, and 3.4 mM, respectively, and is reduced in the presence of dGTP with a Km of 0.13 mM^[5].
Formycin triphosphate partially substitutes for ATP in E. coli RNA polymerase assays, supporting RNA synthesis up to 47-68% on native DNA templates and up to 56%, 94%, and 67% on poly d (A-T), poly d (T-C)·d (A-G), and poly d (T-G)·d (A-C) templates, respectively^[6].
Formycin triphosphate is faithfully incorporated by E. coli RNA polymerase in place of ATP, generating poly (F-U), poly (F-C), and poly (F-G) with alternating FpUp, FpCp, and FpGp sequences^[6].
Formycin triphosphate does not substitute for UTP, CTP, or GTP in E. coli RNA polymerase reactions directed by poly d (T-C)·d (A-G) or poly d (T-G)·d (A-C)^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.