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Cat. No.: HY-161681

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Formycin triphosphate

Formycin triphosphate is a fluorescent ATP analog. Formycin triphosphate is a competitive inhibitor of chicken liver pyruvate carboxylase with respect to ATP. Formycin triphosphate can be used in research on human colon cancer.

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CAS No.: 16409-13-5
화학식: C₁₀H₁₆N₅O₁₃P₃
분자량:507.18

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (280 KB)

Formycin triphosphate Chemical Structure

CAS No.: 16409-13-5

Amount: USD 0.00

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- In Vitro
Chemical Information
순도&문서
References
Calculators
Help & FAQs
Classifications
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Biological Activity

제품 설명

In Vitro

Formycin triphosphate is not reduced in the cell-free Lactobacillus leichmannii ribonucleotide reductase assay in the absence of dGTP, but acts as a linear competitive inhibitor of ATP, GTP, and UTP reduction with Ki values of 0.93, 0.67, and 3.4 mM, respectively, and is reduced in the presence of dGTP with a Km of 0.13 mM^[5].
Formycin triphosphate partially substitutes for ATP in E. coli RNA polymerase assays, supporting RNA synthesis up to 47-68% on native DNA templates and up to 56%, 94%, and 67% on poly d (A-T), poly d (T-C)·d (A-G), and poly d (T-G)·d (A-C) templates, respectively^[6].
Formycin triphosphate is faithfully incorporated by E. coli RNA polymerase in place of ATP, generating poly (F-U), poly (F-C), and poly (F-G) with alternating FpUp, FpCp, and FpGp sequences^[6].
Formycin triphosphate does not substitute for UTP, CTP, or GTP in E. coli RNA polymerase reactions directed by poly d (T-C)·d (A-G) or poly d (T-G)·d (A-C)^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 16409-13-5
분자량 507.18
화학식 C₁₀H₁₆N₅O₁₃P₃
SMILES

NC1=C2C(C([C@@H]3O[C@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[C@@H](O)[C@H]3O)=NN2)=NC=N1
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Glazer RI, et al. Effects of 8-azaadenosine and formycin on cell lethality and the synthesis and methylation of nucleic acids in human colon carcinoma cells in culture. Biochemical pharmacology. 1982 Oct 15;31(20):3207-14.

[2]. Ronjat M, et al. Study of the nucleotide binding site of the yeast Schizosaccharomyces pombe plasma membrane H+-ATPase using formycin triphosphate-terbium complex. The Journal of biological chemistry. 1987 Mar 05;262(7):3146-53.

[3]. Attwood PV, et al. Interaction of formycin A-5'-triphosphate with pyruvate carboxylase. FEBS letters. 1984 Sep 17;175(1):45-50. [Content Brief]

[4]. Henderson JF, et al. Biochemical effects of formycin, an adenosine analog. Cancer Research. 1967 Apr 1;27(4):715-9.

[5]. Brinkley SA, et al. Enzymic preparation of the 5'-triphosphates of 2'-deoxytubercidin, 2'-deoxytoyocamycin, and 2'-deoxyformycin and the allosteric effects of these nucleotides on ribonucleotide reductase. Biochemistry. 2002 May 1;17(12).

[6]. Ikehara M, et al. Synthesis of formycin triphosphate and its incorporation into ribopolynucleotide by DNA‑dependent RNA polymerase. Biochimica et Biophysica Acta. 1968 Jan 29;155(1):82‑90.

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Keywords

MOQ

Minimum order quantity

100 mg

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