Nuclear Fast Red
Based on 1 Customer Validation
Nuclear Fast Red, an anthraquinone dye, is commonly used in conjunction with an excess of aluminum ions as a red nuclear counterstain. Nuclear fast red has been used as a histochemical and colorimetric reagent for calcium. Nuclear fast red as highly sensitive "off/on" fluorescent probe for detecting guanine.
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- Purity: 97.62%
- CAS No.: 6409-77-4
- 화학식: C14H8NNaO7S
- 분자량:357.27
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보관:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
Nuclear Fast Red is a photosensitizer that mediates the single electron transfer, generates reactive oxygen species and other free radicals under visible light irradiation[3].
Nuclear Fast Red spontaneously and irreversibly binds to DNA in the groove structure, and forms a non-fluorescent complex. Nuclear Fast Red can be used as a contrast dye in biological experiments[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 6409-77-4
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Appearance Solid
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분자량 357.27
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화학식 C14H8NNaO7S
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Color Brown to black
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SMILES
O=S(C(C(O)=C1C2=O)=C(O)C(N)=C1C(C3=C2C=CC=C3)=O)(O[Na])=O
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Synonyms
Kernechtrot
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선적
Room temperature in continental US; may vary elsewhere.
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보관
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
용액&용해도
DMSO : 5.83 mg/mL (16.32 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (277 KB)
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SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
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Handling Instructions (2659 KB)
References
[1]. Yi Cui, et al. Nuclear fast red as highly sensitive "off/on" fluorescent probe for detecting guanine. Talanta. 2014 Dec;130:536-41. [Content Brief]
[2]. M Frank , et al. Certification procedures for nuclear fast red (Kernechtrot), CI 60760. Biotech Histochem. 2007 Feb;82(1):35-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7990 mL | 13.9950 mL | 27.9900 mL | 69.9751 mL |
| 5 mM | 0.5598 mL | 2.7990 mL | 5.5980 mL | 13.9950 mL | |
| 10 mM | 0.2799 mL | 1.3995 mL | 2.7990 mL | 6.9975 mL | |
| 15 mM | 0.1866 mL | 0.9330 mL | 1.8660 mL | 4.6650 mL |
Nuclear Fast Red Related Classifications
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