OXA(17-33)
Based on 1 Customer Validation
OXA (17-33) (Orexin A (17-33) (human, mouse, rat, bovine)) is the shortest active orexin peptide that selectively targets OX1 (EC50=8.29 nM), with 23-fold selectivity for the OX1 receptor over the OX2 receptor. The activity of OXA (17-33) depends on the Tyr17, Leu20, Asn25, His26 residues and the spatial conformation of the α-helix. OXA (17-33) activates signaling pathways involving inositol-1,4,5-trisphosphate receptor (IP3R), phospholipase D (PL-D) and choline-Sigma-1R, thereby increasing the cytoplasmic Ca2+ level in nucleus accumbens neurons, an effect that is blocked by Sigma-1R antagonists. OXA (17-33) serves as an important biological probe for investigating the function of the OX1 receptor. OXA (17-33) can be modified via incorporation of mixed disulfide bonds of homocysteine and cysteamine, and is widely used in studies related to insomnia and narcolepsy.
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- Purity: 97.48%
- CAS No.: 343268-91-7
- 화학식: C79H125N23O22
- 분자량:1748.98
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보관:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
All Orexin Receptor (OX Receptor) Isoforms
MoreAll Sigma Receptor Isoforms
MoreAll Phospholipase Isoforms
More
Biological Activity
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OX1 8.29 nM (EC50) |
OX2 187 nM (EC50) |
PLD1 |
Sigma 1 Receptor |
OXA(17-33) (0.1-100 nM) dose-dependently increases cytosolic calcium concentration in cultured nucleus accumbens neurons via activation of OX1 receptors, with a transient response observed at the 10 nM concentration[2].
OXA(17-33) (10 nM) increases cytosolic calcium concentration in cultured nucleus accumbens neurons in Ca2+-free saline via an IP3 receptor-dependent mechanism, with no contribution from endo-lysosomal calcium stores[2].
OXA(17-33) (10 nM) increases cytosolic calcium concentration in cultured nucleus accumbens neurons via a choline-Sigma-1R-dependent mechanism, as inhibition of PLD or antagonism of Sigma-1R reduces the magnitude of the response[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 343268-91-7
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Appearance Solid
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분자량 1748.98
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화학식 C79H125N23O22
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Color White to off-white
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Synonyms
Orexin A (17-33) (human, mouse, rat, bovine)
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Sequence
Tyr-Glu-Leu-Leu-His-Gly-Ala-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr-Leu-NH2
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Sequence Shortening
YELLHGAGNHAAGILTL-NH2
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
용액&용해도
H2O : ≥ 12.5 mg/mL (7.15 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (270 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. German NA, et al. Truncated Orexin Peptides: Structure-Activity Relationship Studies. ACS Med Chem Lett. 2013;4(12):1224-1227. [Content Brief]
[3]. Einsiedel J, et al. Development of disulfide-functionalized peptides covalently binding G protein-coupled receptors. Bioorg Med Chem. 2022;61:116720. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.5718 mL | 2.8588 mL | 5.7176 mL | 14.2940 mL |
| 5 mM | 0.1144 mL | 0.5718 mL | 1.1435 mL | 2.8588 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
OXA(17-33) Related Classifications
HY-P1341 Related Classifications
Powered by Bioz
- OXA(17-33)
- 343268-91-7
- Orexin A (17-33) (human, mouse, rat, bovine)
- Orexin Receptor (OX Receptor)
- Sigma Receptor
- Phospholipase
- G protein-coupled receptor
- OX1 receptor
- Sigma-1R antagonists
- phospholipase D
- nucleus accumbens neurons
- CHO-RD-HGA16 cells
- inositol-1,4,5-trisphosphate receptors
- orexin peptide
- OX2 receptors
- choline-Sigma-1R
- Inhibitor
- inhibitor
- inhibit