ST-401
Based on 1 Customer Validation
ST-401, a microtubule-targeting agent (MTA), is a brain-penetrant microtubule (MT) assembly inhibitor. ST-401 disrupts microtubule (MT) function through gentle and reverisible reduction in MT assembly that triggers mitotic delay and cell death in interphase. ST-401 shows a potent antitumor activity.
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- Purity: 99.41%
- CAS No.: 2419942-78-0
- 화학식: C24H20N2O
- 분자량:352.43
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
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Biological Activity
ST-401 is a hybrid molecule that combines a secondary alcohol and quinoline in one structure. ST-401 competes for 89% of [3H]colchicine binding to tubulin. In a turbidity assay, ST-401 inhibits tubulin assembly with an IC50 of 1.1 µM. In a pelleting assay that measures release of free tubulin, ST-401 inhibits tubulin assembly with comparable IC50 value of 3.2 µM[1].
ST-401 exhibits similar antitumor activities in the low nanomolar range in most human cancer cell lines, including the six glioma lines (SF-268, SF-295, SF-539, SNB-19, SNB-75, and U251: IC50s 23-69 nM)[1].
ST-401 shows the antitumor activity in PD-glioma is in the low nanomolar (IC50: 24-47 nM)[1].
ST-401 (72 h) reduces overall cell viability in MGG8 and T98G cells with comparable IC50s (14 and 36 nM, respectively)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Nude mice (10-14 weeks of age) injection with COLO205 cells[1]
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Dosage:20 mg/kg
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Administration:i.p; bidaily; 5 days/week for 4 weeks
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Result:Resulted in significant antitumor activity.
Chemical Information
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CAS No. 2419942-78-0
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Appearance Solid
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분자량 352.43
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화학식 C24H20N2O
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Color White to light yellow
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SMILES
OC(C1=CC=CC2=C1C=CC=N2)C3=CC4=C(C=C3)N(CC)C5=CC=CC=C54
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
DMSO : 100 mg/mL (283.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (14.19 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (278 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8374 mL | 14.1872 mL | 28.3744 mL | 70.9361 mL |
| 5 mM | 0.5675 mL | 2.8374 mL | 5.6749 mL | 14.1872 mL | |
| 10 mM | 0.2837 mL | 1.4187 mL | 2.8374 mL | 7.0936 mL | |
| 15 mM | 0.1892 mL | 0.9458 mL | 1.8916 mL | 4.7291 mL | |
| 20 mM | 0.1419 mL | 0.7094 mL | 1.4187 mL | 3.5468 mL | |
| 25 mM | 0.1135 mL | 0.5675 mL | 1.1350 mL | 2.8374 mL | |
| 30 mM | 0.0946 mL | 0.4729 mL | 0.9458 mL | 2.3645 mL | |
| 40 mM | 0.0709 mL | 0.3547 mL | 0.7094 mL | 1.7734 mL | |
| 50 mM | 0.0567 mL | 0.2837 mL | 0.5675 mL | 1.4187 mL | |
| 60 mM | 0.0473 mL | 0.2365 mL | 0.4729 mL | 1.1823 mL | |
| 80 mM | 0.0355 mL | 0.1773 mL | 0.3547 mL | 0.8867 mL | |
| 100 mM | 0.0284 mL | 0.1419 mL | 0.2837 mL | 0.7094 mL |
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