Tositumomab
Based on 1 Customer Validation
Tositumomab (Anti-Human CD20 Recombinant Antibody) is a mouse-derived IgG2a monoclonal antibody, targeting to human CD20. Tositumomab can deplete B cells. Tositumomab can be used for the research of cancer, such as lymphoma.
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- Purity: 96.00%
- CAS No.: 192391-48-3
- 분자량:147.298 kDa
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Mouse IgG2a
Human
MS4A1
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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IgG2a-lambda
ELISA, FACS, Functional assay
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Flow Cytometry analysis of Raji cells labelling CD20/MS4A1 (red) with Tositumomab (anti-CD20/MS4A1) (HY-P99326). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG2a (HY-P99978, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black). -
Loaded Tositumomab on AHC2 biosensor, can bind CD20/MS4A1 Protein, Human (Trx-His, HY-P7896A) with an affinity constant of <1.000E-11 M as determined in BLI assay.
Chemical Information
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CAS No. 192391-48-3
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Appearance Liquid
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분자량 147.298 kDa
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Color Colorless to light yellow
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SMILES
[Tositumomab]
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Synonyms
Anti-Human CD20 Recombinant Antibody; V10XA53
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선적
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
순도&문서
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Data Sheet (257 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Havlena GT, et al. Cure of Micrometastatic B-Cell Lymphoma in a SCID Mouse Model Using 213Bi-Anti-CD20 Monoclonal Antibody. J Nucl Med. 2023 Jan;64(1):109-116. [Content Brief]
[2]. Beers SA, et al. Type II (tositumomab) anti-CD20 monoclonal antibody out performs type I (rituximab-like) reagents in B-cell depletion regardless of complement activation. Blood. 2008 Nov 15;112(10):4170-7. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)