Kumujian A
Based on 1 Customer Validation
Kumujian A (1-Ethoxycarbonyl-β-carboline), an anti-inflammatory agent, inhibits both superoxide anion generation (IC50 = 4.87 μg/mL) and elastase release (IC50 = 6.29 μg/mL).
For research use only. We do not sell to patients.
- Purity : 98.32%
- CAS No.: 72755-19-2
- Formula: C14H12N2O2
- Molecular Weight:240.26
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Neutrophil | IC50 |
4.87 μg/mL
Compound: 3
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Antiinflammatory activity in FMLP/CB-induced human neutrophils assessed as inhibition of superoxide anion generation
Antiinflammatory activity in FMLP/CB-induced human neutrophils assessed as inhibition of superoxide anion generation
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[PMID: 26248804] |
| Neutrophil | IC50 |
6.29 μg/mL
Compound: 3
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Antiinflammatory activity in FMLP/CB-induced human neutrophils assessed as inhibition of elastase release
Antiinflammatory activity in FMLP/CB-induced human neutrophils assessed as inhibition of elastase release
|
[PMID: 26248804] |
Chemical Information
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CAS No. 72755-19-2
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Appearance Solid
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Molecular Weight 240.26
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Formula C14H12N2O2
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Color Light yellow to orange
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SMILES
O=C(C1=NC=CC2=C1NC3=C2C=CC=C3)OCC
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Synonyms
1-Ethoxycarbonyl-β-carboline
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (416.22 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Research Protocol for Inflammation-related Diseases
The NLRP3 inflammasome is a cytosolic innate immune signaling platform that integrates priming signals and danger-signal activation to promote caspase-1 activation, maturation of IL-1β and IL-18, and gasdermin D-mediated pyroptotic cell death. The core experimental logic is to determine whether inflammatory disease phenotypes are driven by increased NLRP3 expression, ASC-containing inflammasome assembly, caspase-1 cleavage, GSDMD cleavage, and extracellular release of IL-1β/IL-18 rather than by nonspecific cell injury alone. The pathway is strongly linked to inflammation-related disease phenotypes because monosodium urate crystals activate NALP3/NLRP3 inflammasome signaling in gout-like crystal inflammation, cholesterol crystals activate NLRP3 inflammasomes in atherogenesis models, and DSS-induced intestinal inflammation has been reported to involve NLRP3 inflammasome activity. However, experimental colitis studies also show context-dependent protective effects of NLRP3 inflammasome co
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.1622 mL | 20.8108 mL | 41.6216 mL | 104.0539 mL |
| 5 mM | 0.8324 mL | 4.1622 mL | 8.3243 mL | 20.8108 mL | |
| 10 mM | 0.4162 mL | 2.0811 mL | 4.1622 mL | 10.4054 mL | |
| 15 mM | 0.2775 mL | 1.3874 mL | 2.7748 mL | 6.9369 mL | |
| 20 mM | 0.2081 mL | 1.0405 mL | 2.0811 mL | 5.2027 mL | |
| 25 mM | 0.1665 mL | 0.8324 mL | 1.6649 mL | 4.1622 mL | |
| 30 mM | 0.1387 mL | 0.6937 mL | 1.3874 mL | 3.4685 mL | |
| 40 mM | 0.1041 mL | 0.5203 mL | 1.0405 mL | 2.6013 mL | |
| 50 mM | 0.0832 mL | 0.4162 mL | 0.8324 mL | 2.0811 mL | |
| 60 mM | 0.0694 mL | 0.3468 mL | 0.6937 mL | 1.7342 mL | |
| 80 mM | 0.0520 mL | 0.2601 mL | 0.5203 mL | 1.3007 mL | |
| 100 mM | 0.0416 mL | 0.2081 mL | 0.4162 mL | 1.0405 mL |
Kumujian A Related Classifications
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