L-Dihydroorotic acid
Based on 4 publication(s) in Google Scholar
L-Dihydroorotic acid is an important intermediate in the metabolism of orotic acid and a substrate of dihydroorotate dehydrogenase (DHODH). L-Dihydroorotic acid can reversibly hydrolyze to yield the acyclic L-ureidosuccinic acid by dihydrowhey enzyme.
For research use only. We do not sell to patients.
- Purity: 99.97%
- CAS No.: 5988-19-2
- Formula: C5H6N2O4
- Molecular Weight:158.11
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) L-Dihydroorotic acid
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
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Microbial Metabolite |
Human Endogenous Metabolite |
Orotic acid is reversibly converted to L-Dihydroorotic acid under the catalysis of DHODH and with the participation of DPNH (reduced nicotinamide adenine dinucleotide) and H⁺. This reaction is a key step in the synthesis and decomposition of pyrimidines and is likely to be widespread in tissues such as microorganisms and animal livers[1][2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 5988-19-2
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Appearance Solid
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Molecular Weight 158.11
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Formula C5H6N2O4
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Color White to light yellow
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SMILES
O=C([C@H](CC(N1)=O)NC1=O)O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (4)
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Journal Impact Factor
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Most Recent
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Autophagy
2026 Jul;22(7):1657-1678. PMID: 41968644 -
EMBO Mol Med
De novo pyrimidine synthesis is a collateral metabolic vulnerability in NF2-deficient mesothelioma. [Abstract]2025 Jul 24. PMID: 40707702 -
Pestic Biochem Physiol
Plumbagin targets dihydroorotase in Magnaporthe oryzae to inhibit infection and shows antifungal activity against multiple phytopathogenic fungi. [Abstract]2026 Jun:221:107096. PMID: 42264707 -
Solvent & Solubility
DMF : 33.33 mg/mL (210.80 mM; Need ultrasonic)
DMSO : 25 mg/mL (158.12 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (31.62 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (15.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (15.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% DMF 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (15.81 mM); Clear solution
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 7.69 mg/mL (48.64 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
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- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO / DMF | 1 mM | 6.3247 mL | 31.6236 mL | 63.2471 mL | 158.1178 mL |
| 5 mM | 1.2649 mL | 6.3247 mL | 12.6494 mL | 31.6236 mL | |
| 10 mM | 0.6325 mL | 3.1624 mL | 6.3247 mL | 15.8118 mL | |
| 15 mM | 0.4216 mL | 2.1082 mL | 4.2165 mL | 10.5412 mL | |
| 20 mM | 0.3162 mL | 1.5812 mL | 3.1624 mL | 7.9059 mL | |
| 25 mM | 0.2530 mL | 1.2649 mL | 2.5299 mL | 6.3247 mL | |
| 30 mM | 0.2108 mL | 1.0541 mL | 2.1082 mL | 5.2706 mL | |
| DMSO / DMF | 40 mM | 0.1581 mL | 0.7906 mL | 1.5812 mL | 3.9529 mL |
| 50 mM | 0.1265 mL | 0.6325 mL | 1.2649 mL | 3.1624 mL | |
| 60 mM | 0.1054 mL | 0.5271 mL | 1.0541 mL | 2.6353 mL | |
| 80 mM | 0.0791 mL | 0.3953 mL | 0.7906 mL | 1.9765 mL | |
| 100 mM | 0.0632 mL | 0.3162 mL | 0.6325 mL | 1.5812 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
L-Dihydroorotic acid Related Classifications
HY-W015495 Related Classifications
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