MDX-1401 

MDX-1401 is a fully human IgG1 (κ isotype) monoclonal antibody against CD30, with a K_d value of 0.83 nmol/L. MDX-1401 induces antibody-dependent cellular cytotoxicity in CD30-expressing cells and inhibits tumor growth in CD30⁺ lymphoma xenograft models. MDX-1401 can be used in research related to malignant lymphoma and Hodgkin lymphoma^[1][2].

Human

MDX-1401 binds to recombinant CD30 with a K_d value of 0.83 nmol/L, exhibiting binding affinity comparable to that of its parental antibody MDX-060^[1].
MDX-1401 (¹²⁵I-MDX-1401) binds to CD30 on the surface of the Hodgkin's lymphoma cell line L540 cells, with a K_d value of approximately 1.9 nmol/L^[1].
MDX-1401 (0.012-50 μg/mL; 30 min primary incubation, 30 min secondary incubation) binds to CD30⁺ lymphoma cell lines (L540, Karpas-299, L428, L1236) in a dose-dependent manner, and its binding properties are consistent with those of the parental antibody MDX-060^[1].
MDX-1401 mediates FcγRIIIa-dependent antibody-dependent cellular cytotoxicity (ADCC) against L540 Hodgkin lymphoma cells, with an EC₅₀ of 0.009 μg/mL and a maximum cell lysis rate of 87%, exhibiting superior potency and efficacy compared to the parental antibody MDX-060^[1].
MDX-1401 (treated at 37°C for 4 h) mediates dose-dependent ADCC against CD30⁺ lymphoma cell lines (L540, Karpas-299, L428, L1236), with EC₅₀ values ranging from 0.008 to 0.04 μg/mL and maximum lysis rates of 13% to 68%. This includes activity against L1236 cells with low CD30 expression, against which the parental antibody MDX-060 shows no activity^[1].
MDX-1401 binds to CD30 on L540 Hodgkin lymphoma cells with a K_D value of 1.9 nM, and its antigen-binding affinity is nearly identical to that of its parental antibody MDX-060 (K_D ~1.4 nM)^[2].
MDX-1401 mediates significantly stronger and more effective ADCC against CD30-expressing tumor cell lines (including those with low CD30 expression) across all FcgRIIIa polymorphic variants, with a decreased EC₅₀ and an increased maximum specific lysis rate compared to its parental antibody^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MDX-1401 (1 mg/kg; i.p.; single dose) significantly prolongs the survival of SCID mice with systemic Karpas-299 ALCL^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

943  [NCBI]

P28908

TNFRSF8/CD30

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Cardarelli PM, et al. In vitro and in vivo characterization of MDX-1401 for therapy of malignant lymphoma. Clin Cancer Res. 2009;15(10):3376-3383.

  [2]. Cardarelli P M, et al. Characterization of MDX-1401, a Human Anti-CD30 Antibody with Enhanced Effector Function, for Therapy of Malignant Lymphoma[J]. Blood, 2008, 112(11): 1580.

  [3]. Foyil KV, et al. Anti-CD30 Antibodies for Hodgkin lymphoma. Curr Hematol Malig Rep. 2010;5(3):140-147.