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Cat. No.: HY-151758

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Methyltetrazine-PEG12-NHS ester

Methyltetrazine-PEG12-NHS ester is a click chemistry reagent containing an azide group. Methyltetrazine-PEG12-NHS ester reacts with compounds containing TCO to form stable covalent bonds.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

화학식: C₃₈H₅₉N₅O₁₆
분자량:841.90

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (267 KB)

Methyltetrazine-PEG12-NHS ester Chemical Structure

Amount: USD 0.00

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Use of MCE Products

One-Stop Platform：From Independent R&D to Pilot-Scale Production

Biological Activity

제품 설명

Methyltetrazine-PEG12-NHS ester is a click chemistry reagent containing an azide group. Methyltetrazine-PEG12-NHS ester reacts with compounds containing TCO to form stable covalent bonds^[1].

All ADC Linkers Isoforms :

Chemical Information

분자량 841.90
화학식 C₃₈H₅₉N₅O₁₆
SMILES

CC1=NN=C(C2=CC=C(OCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCC(ON3C(CCC3=O)=O)=O)C=C2)N=N1
선적

Room temperature in continental US; may vary elsewhere.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocol

All Cell Biology

EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (267 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Biedka S, et, al. Reversible Click Chemistry Tag for Universal Proteome Sample Preparation for Top-Down and Bottom-Up Analysis. J Proteome Res. 2021 Oct 1;20(10):4787-4800. [Content Brief]

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Keywords

Methyltetrazine-PEG12-NHS ester

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Minimum order quantity

100 mg

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Amount: USD 0.00

Biological Activity
- 제품 설명
Chemical Information
Protocol
순도&문서
References

상품명:
Cat. No.:
수량:
MCE Japan Authorized Agent:

상품명:
CAS No.:
Species:
Purity:
수량:

Methyltetrazine-PEG12-NHS ester

All ADC Linkers Isoforms

Biological Activity

제품 설명

Chemical Information

Protocol

순도&문서

References

Calculators

Help & FAQs

Methyltetrazine-PEG12-NHS ester Related Classifications

HY-151758 Related Classifications

Bioz

Keywords

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