Monochlorobimane (solution) 

Monochlorobimane (Chlorobimane) (solution) is a fluorescent dye (λ_ex=380 nm, λ_em=470 nm) to measure glutathione (GSH) in cellular assays^[1].
Solvent and concentration: DMSO: 10 mM

Guide (The following is a recommended experimental protocol for reference only and should be adjusted according to specific needs)
1. Solution preparation^[3]
1.1 Stock solution storage
Storage: Aliquot and store protected from light at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
1.2 Working solution preparation
Dilute with PBS or cell culture medium. The stock solution can be diluted as needed according to actual conditions. Note: if the solvent is DMSO, its cytotoxicity must be considered and a solvent control should be prepared; if the solvent is pure water, the working solution should be filtered and sterilized before being added to cells.
Note: The working solution should be freshly prepared, used immediately, and protected from light.

2. Cell staining
2.1 Seed cells into black 96-well microplates with clear bottoms.
2.2 Add Monochlorobimane (1-10 μM) and incubate for 30 min (optimize according to the experiment).
2.3 Measure fluorescence intensity using a fluorescence microscope or a fluorescence microplate reader (Ex/Em = 380/480 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

226.66

C₁₀H₁₁ClN₂O₂

76421-73-3

O=C1N(C(C(C)=C2C)=O)N2C(CCl)=C1C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Kamencic H, et al. Monochlorobimane fluorometric method to measure tissue glutathione. Anal Biochem. 2000 Nov 1;286(1):35-7.  [Content Brief]

  [2]. Manuela D. Machado, et al. Assessment of cellular reduced glutathione content in Pseudokirchneriella subcapitata using monochlorobimane. Journal of Applied Phycology volume 24, pages1509–1516(2012).

  [3]. Fotopoulou T, et al. Design and Synthesis of Novel Antioxidant 2-Substituted-5,7,8-Trimethyl-1,4-Benzoxazine Hybrids: Effects on Young and Senescent Fibroblasts. Antioxidants (Basel). 2024 Jun 29;13(7):798.  [Content Brief]