Mutanolysin 

Mutanolysin is a bacteriolytic agent. Mutanolysin is a muralytic enzyme that can prevent hepatic injury. Mutanolysin can digest the cell wall of S. mutans BHT and shows antibacterial activity. Mutanolysin reduces TNF-α production in isolated Kupffer cells stimulated with peptidoglycan-polysaccharide (PG-APS). Mutanolysin can be used for the researches of infection, inflammation and hepatic injury^[1][2][3].

Mutanolysin (200 μg; 36 h) digests PG-APS to reduce TNFα production by purified Lewis rat Kupffer cells by 75%, from 486 pg/mL to 145 pg/mL^[1].
Mutanolysin (20 μg/mL, 90 min) lyses and kills live S. mutans AHT and BHT cells after 90 min of incubation, with 99.999% killing efficacy against S. mutans AHT and 99.0% killing efficacy against S. mutans BHT^[2].
Mutanolysin exhibits broad lytic activity against gram-positive oral pathogens including S. mutans, A. viscosus, and L. acidophilus, but is inactive against S. aureus, gram-negative bacteria, and yeast^[2].
Mutanolysin lyses S. mutans BHT cells by rupturing cell walls^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Mutanolysin (200 μg; i.v.; three times per week; within 24 h of SFBL surgery for 4-8 weeks) prevents small bowel bacterial overgrowth-induced hepatobiliary injury in female Lewis rats^[1].
Mutanolysin (0.4 mg; i.v.; single dose) completely prevents the development of chronic erosive arthritis, resolves acute arthritis within 14 days, and eliminates granulomatous lesions in visceral tissues^[3].
Mutanolysin (0.4 mg; i.v.; single dose; administered 24 h or 3 days post-PG-APS) reduces acute arthritis severity and completely prevents the development of chronic erosive arthritis^[3].
Mutanolysin (0.4 mg; i.v.; single dose; administered 14 days post-PG-APS) significantly reduces the severity of chronic joint disease, with only 33% of treated rats developing recurrent disease^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

55466-22-3

Solid

White to off-white

[Mutanolysin]

≥ 4000 U/mg protein

One unit is defined as the amount of enzyme that will produce a change in absorbance at 600 nm of 0.01 per minute at pH 6.0 at 37°C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Lichtman SN, et al. Degradation of endogenous bacterial cell wall polymers by the muralytic enzyme mutanolysin prevents hepatobiliary injury in genetically susceptible rats with experimental intestinal bacterial overgrowth. J Clin Invest. 1992;90(4):1313-1322.  [Content Brief]

  [2]. Yokogawa K, et al. Mutanolysin, bacteriolytic agent for cariogenic Streptococci: partial purification and properties. Antimicrob Agents Chemother. 1974;6(2):156-165.  [Content Brief]

  [3]. Janusz MJ, et al. Treatment of experimental erosive arthritis in rats by injection of the muralytic enzyme mutanolysin. J Exp Med. 1984;160(5):1360-1374.