BPBA
Based on 1 Customer Validation
BPBA (2-Bromopyridine-5-boronic acid) is a derivatization reagent derived from Boric acid (HY-Y0314) with enhanced ionization efficiency in positive-mode electrospray ionization mass spectrometry. BPBA forms boronate ester derivatives with vicinal diols. It enables rapid, catalyst-free labeling operations, improves the detection sensitivity of liquid chromatography/mass spectrometry, and reduces false positive errors.
For research use only. We do not sell to patients.
- Purity: 99.85%
- CAS No.: 223463-14-7
- Formula: C5H5BBrNO2
- Molecular Weight:201.82
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
BPBA rapidly derivatizes Epibrassinolide (HY-N0848), Homobrassinolide (HY-139842), and 28-epihomoBR under catalyst-free, room-temperature conditions to form stable products[1].
BPBA derivatization enables highly sensitive LC/ESI-MS detection of vicinal diols[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 223463-14-7
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Appearance Solid
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Molecular Weight 201.82
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Formula C5H5BBrNO2
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Color White to off-white
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SMILES
OB(C1=CC=C(Br)N=C1)O
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Synonyms
2-Bromopyridine-5-boronic acid
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (495.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.39 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.39 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Huo F, et al. A new derivatization approach for the rapid and sensitive analysis of brassinosteroids by using ultra high performance liquid chromatography-electrospray ionization triple quadrupole mass spectrometry. Talanta. 2012 Sep 15;99:420-5. [Content Brief]
[2]. Shen S, et al. An approach based on liquid chromatography/electrospray ionization-mass spectrometry to detect diol metabolites as biomarkers of exposure to styrene and 1,3-butadiene. Analytical biochemistry. 2009 Mar 15;386(2):186-93. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.9549 mL | 24.7746 mL | 49.5491 mL | 123.8728 mL |
| 5 mM | 0.9910 mL | 4.9549 mL | 9.9098 mL | 24.7746 mL | |
| 10 mM | 0.4955 mL | 2.4775 mL | 4.9549 mL | 12.3873 mL | |
| 15 mM | 0.3303 mL | 1.6516 mL | 3.3033 mL | 8.2582 mL | |
| 20 mM | 0.2477 mL | 1.2387 mL | 2.4775 mL | 6.1936 mL | |
| 25 mM | 0.1982 mL | 0.9910 mL | 1.9820 mL | 4.9549 mL | |
| 30 mM | 0.1652 mL | 0.8258 mL | 1.6516 mL | 4.1291 mL | |
| 40 mM | 0.1239 mL | 0.6194 mL | 1.2387 mL | 3.0968 mL | |
| 50 mM | 0.0991 mL | 0.4955 mL | 0.9910 mL | 2.4775 mL | |
| 60 mM | 0.0826 mL | 0.4129 mL | 0.8258 mL | 2.0645 mL | |
| 80 mM | 0.0619 mL | 0.3097 mL | 0.6194 mL | 1.5484 mL | |
| 100 mM | 0.0495 mL | 0.2477 mL | 0.4955 mL | 1.2387 mL |
BPBA Related Classifications
HY-W014815 Related Classifications
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