N1-Methylpseudouridine-5′-triphosphate solution (100 mM) (Synonyms: 1-Methylpseudouridine-5′-triphosphate)

Cat. No.: HY-112582A Purity: 99.70%: Data Sheet
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N1-Methylpseudouridine-5′-triphosphate (1-Methylpseudouridine-5′-triphosphate) is a nucleobase-modified nucleotide, used for synthesizing mRNA with reduced immunogenicity and improved stability.

For research use only. We do not sell to patients.

N1-Methylpseudouridine-5′-triphosphate solution (100 mM) Chemical Structure

CAS No. : 1428903-59-6

Size	Stock	Quantity
Solvent
100 μL	In-stock	Estimated Time of Arrival: December 31
Solvent
200 μL	In-stock	Estimated Time of Arrival: December 31
Solvent
500 μL	In-stock	Estimated Time of Arrival: December 31
Solvent
1 mL	In-stock	Estimated Time of Arrival: December 31
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1 Publications Citing Use of MCE N1-Methylpseudouridine-5′-triphosphate solution (100 mM)

•ACS Nano. 2026 Mar 17.

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Description	N1-Methylpseudouridine-5′-triphosphate (1-Methylpseudouridine-5′-triphosphate) is a nucleobase-modified nucleotide, used for synthesizing mRNA with reduced immunogenicity and improved stability.
In Vitro	N1-Methylpseudouridine-5′-triphosphate is used to synthesize mRNAs with lower immunogenicity and higher stability^[1]. Modified mRNAs synthesized from N1-Methylpseudouridine-5′-triphosphate can be delivered to the heart^[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. N1-Methylpseudouridine-5′-triphosphate solution (100 mM) Related Antibodies
Molecular Weight	498.17
Formula	C₁₀H₁₇N₂O₁₅P₃
CAS No.	1428903-59-6
Appearance	Liquid
Color	Colorless to light yellow
SMILES	O[C@H]1[C@@H](O)[C@H](C(C(N2)=O)=CN(C)C2=O)O[C@@H]1COP(OP(OP(O)(O)=O)(O)=O)(O)=O
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	Solution, -20°C, 2 years
Solvent & Solubility	In Vitro: H₂O : ≥ 100 mg/mL (200.73 mM) "≥" means soluble, but saturation unknown. Molarity Calculator Dilution Calculator Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Mass = Concentration × Volume × Molecular Weight Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final) This equation is commonly abbreviated as: C₁V₁ = C₂V₂ Concentration _(start) C1 × Volume _(start) V1 = Concentration _(final) C2 × Volume _(final) V2 In Vivo Dissolution Calculator Please enter the basic information of animal experiments: Dosage mg/kg Animal weight (per animal) g Dosing volume (per animal) μL Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments. Calculation results: Working solution concentration: mg/mL This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details. The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE). Tel: 609-228-6898 E-mail: [email protected] Technical Support: [email protected]
Purity & Documentation	Purity: 99.88% Select Batch: Data Sheet (275 KB) SDS (394 KB) COA (253 KB) HNMR (263 KB) RP-HPLC (167 KB) MS (70 KB) Handling Instructions (2659 KB)
References	[1]. Andries O, Mc Cafferty S, De Smedt SC, Weiss R, Sanders NN, Kitada T. N(1)-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice. J Control Release. 2015;217:337-344. [Content Brief] [2]. Sultana N, et al. Optimizing Cardiac Delivery of Modified mRNA. Mol Ther. 2017 Jun 7;25(6):1306-1315. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.0073 mL	10.0367 mL	20.0735 mL	50.1837 mL
	5 mM	0.4015 mL	2.0073 mL	4.0147 mL	10.0367 mL
	10 mM	0.2007 mL	1.0037 mL	2.0073 mL	5.0184 mL
	15 mM	0.1338 mL	0.6691 mL	1.3382 mL	3.3456 mL
	20 mM	0.1004 mL	0.5018 mL	1.0037 mL	2.5092 mL
	25 mM	0.0803 mL	0.4015 mL	0.8029 mL	2.0073 mL
	30 mM	0.0669 mL	0.3346 mL	0.6691 mL	1.6728 mL
	40 mM	0.0502 mL	0.2509 mL	0.5018 mL	1.2546 mL
	50 mM	0.0401 mL	0.2007 mL	0.4015 mL	1.0037 mL
	60 mM	0.0335 mL	0.1673 mL	0.3346 mL	0.8364 mL
	80 mM	0.0251 mL	0.1255 mL	0.2509 mL	0.6273 mL
	100 mM	0.0201 mL	0.1004 mL	0.2007 mL	0.5018 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

N1-Methylpseudouridine-5′-triphosphate solution (100 mM) Related Classifications

Cardiovascular Disease Others

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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N1-Methylpseudouridine-5′-triphosphate solution (100 mM)1428903-59-61-Methylpseudouridine-5′-triphosphateNucleoside Antimetabolite/AnalogDNA/RNA Synthesisnucleobase-modified nucleotidemRNAInhibitorinhibitorinhibit

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N1-Methylpseudouridine-5′-triphosphate solution (100 mM) (Synonyms: 1-Methylpseudouridine-5′-triphosphate)

Customer Review

Other Forms of N1-Methylpseudouridine-5′-triphosphate solution (100 mM):

N1-Methylpseudouridine-5′-triphosphate solution (100 mM) Related Antibodies

1 Publications Citing Use of MCE N1-Methylpseudouridine-5′-triphosphate solution (100 mM)

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All DNA/RNA Synthesis Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

N1-Methylpseudouridine-5′-triphosphate solution (100 mM) Related Antibodies

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Complete Stock Solution Preparation Table

N1-Methylpseudouridine-5′-triphosphate solution (100 mM) Related Classifications

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