1. Cell Cycle/DNA Damage
  2. Nucleoside Antimetabolite/Analog
    DNA/RNA Synthesis
  3. N1-Methylpseudouridine

N1-Methylpseudouridine  (Synonyms: 1-Methylpseudouridine; N1-methyl-pseudouridine)

Cat. No.: HY-112582 Purity: 99.98%
COA Handling Instructions

N1-methyl-pseudouridine (1-Methylpseudouridine), a methylpseudouridine, outperforms 5 mC and 5 mC/N1-methyl-pseudouridine in translation. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density.

For research use only. We do not sell to patients.

N1-Methylpseudouridine Chemical Structure

N1-Methylpseudouridine Chemical Structure

CAS No. : 13860-38-3

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Based on 1 publication(s) in Google Scholar

Other Forms of N1-Methylpseudouridine:

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Description

N1-methyl-pseudouridine (1-Methylpseudouridine), a methylpseudouridine, outperforms 5 mC and 5 mC/N1-methyl-pseudouridine in translation. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density[1].

In Vitro

Incorporation of N1-methyl-pseudouridine into mRNA modifies mRNAs produced higher amounts of luc than the standard Luc mRNA in HEK293T cells. Incorporation of N1-methyl-pseudouridine nucleoside modification in both Luc and GFP mRNA enhances the initiation step of translation, in part by suppressing eIF2α phosphorylation. In addition, polysome formation and growth on the NN1-methyl-pseudouridine-containing Luc mRNA is enhanced due to the reduction of elongation rate. In all the in vitro translation systems, incorporation of N1-methyl-pseudouridine in Luc and GFP mRNAs dramatically enhanced translation. The N1-methyl-pseudouridine-Luc mRNA is associated with heavier polysomes than Luc mRNA[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

N1-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice[2].
N1-methyl-pseudouridine (1-Methylpseudouridine) (20 μg; I.m. or i.d. routes for 21 days) and m5C/ N1-methyl-pseudouridine-modified mRNA respectively have a higher translational capacity than Ψ and m5C/Ψ-modified mRNA in vivo[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 7-week-old Balb/c mice[1]
Dosage: 20 μg
Administration: I.m. or i.d. routes for 21 days
Result: had a higher translational capacity.
Molecular Weight

258.23

Appearance

Solid

Formula

C10H14N2O6

CAS No.
SMILES
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (484.06 mM; Need ultrasonic)

H2O : 50 mg/mL (193.63 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8725 mL 19.3626 mL 38.7252 mL
5 mM 0.7745 mL 3.8725 mL 7.7450 mL
10 mM 0.3873 mL 1.9363 mL 3.8725 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (193.63 mM); Clear solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (8.05 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (8.05 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (8.05 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.98%

References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
N1-Methylpseudouridine
Cat. No.:
HY-112582
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