N3-PEG3-C2-PFP ester
Based on 1 Customer Validation
N3-PEG3-C2-PFP ester is a nonclaevable 3-unit PEG linker used in the synthesis of antibody-drug conjugates (ADCs). N3-PEG3-C2-PFP ester is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
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- Pureté : 95.20%
- CAS No.: 1807530-07-9
- Formule: C15H16F5N3O5
- Masse moléculaire:413.30
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Stockage:Pure form -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
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Help & FAQs
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Activité biologique
Description
IC50 & Target
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Non-cleavable Linker |
Chemical Information
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CAS No. 1807530-07-9
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Appearance Liquid
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Masse moléculaire 413.30
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Formule C15H16F5N3O5
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Color Colorless to light yellow
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SMILES
O=C(OC1=C(F)C(F)=C(F)C(F)=C1F)CCOCCOCCOCCN=[N+]=[N-]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Pure form -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (241.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Pureté et documentation
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Fiche technique (239 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.4196 mL | 12.0978 mL | 24.1955 mL | 60.4888 mL |
| 5 mM | 0.4839 mL | 2.4196 mL | 4.8391 mL | 12.0978 mL | |
| 10 mM | 0.2420 mL | 1.2098 mL | 2.4196 mL | 6.0489 mL | |
| 15 mM | 0.1613 mL | 0.8065 mL | 1.6130 mL | 4.0326 mL | |
| 20 mM | 0.1210 mL | 0.6049 mL | 1.2098 mL | 3.0244 mL | |
| 25 mM | 0.0968 mL | 0.4839 mL | 0.9678 mL | 2.4196 mL | |
| 30 mM | 0.0807 mL | 0.4033 mL | 0.8065 mL | 2.0163 mL | |
| 40 mM | 0.0605 mL | 0.3024 mL | 0.6049 mL | 1.5122 mL | |
| 50 mM | 0.0484 mL | 0.2420 mL | 0.4839 mL | 1.2098 mL | |
| 60 mM | 0.0403 mL | 0.2016 mL | 0.4033 mL | 1.0081 mL | |
| 80 mM | 0.0302 mL | 0.1512 mL | 0.3024 mL | 0.7561 mL | |
| 100 mM | 0.0242 mL | 0.1210 mL | 0.2420 mL | 0.6049 mL |
N3-PEG3-C2-PFP ester Related Classifications
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